Transfer of the methicillin resistance genomic island among staphylococci by conjugation

Molecular Microbiology
M D RayG L Archer

Abstract

Methicillin resistance creates a major obstacle for treatment of Staphylococcus aureus infections. The resistance gene, mecA, is carried on a large (20 kb to > 60 kb) genomic island, staphylococcal cassette chromosome mec (SCCmec), that excises from and inserts site-specifically into the staphylococcal chromosome. However, although SCCmec has been designated a mobile genetic element, a mechanism for its transfer has not been defined. Here we demonstrate the capture and conjugative transfer of excised SCCmec. SCCmec was captured on pGO400, a mupirocin-resistant derivative of the pGO1/pSK41 staphylococcal conjugative plasmid lineage, and pGO400::SCCmec (pRM27) was transferred by filter-mating into both homologous and heterologous S. aureus recipients representing a range of clonal complexes as well as S. epidermidis. The DNA sequence of pRM27 showed that SCCmec had been transferred in its entirety and that its capture had occurred by recombination between IS257/431 elements present on all SCCmec types and pGO1/pSK41 conjugative plasmids. The captured SCCmec excised from the plasmid and inserted site-specifically into the chromosomal att site of both an isogenic S. aureus and a S. epidermidis recipient. These studies describe a me...Continue Reading

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Citations

Jul 19, 2016·World Journal of Emergency Surgery : WJES·Massimo SartelliPerluigi Viale
Oct 19, 2016·Trends in Microbiology·Paul J PlanetBarry Kreiswirth
Oct 6, 2016·Nature Structural & Molecular Biology·Joshua P Ramsay
Jul 25, 2017·Scientific Reports·Nathalie T Reichmann, Mariana G Pinho
Jan 30, 2019·Nature Microbiology·Jakob T Rostøl, Luciano A Marraffini
Aug 3, 2016·Annual Review of Microbiology·J Ross Fitzgerald, Matthew T G Holden
Aug 23, 2019·Foodborne Pathogens and Disease·Arne Schnitt, Bernd-Alois Tenhagen
Apr 5, 2021·Journal of Applied Microbiology·A G Al-BakriK Becker
Mar 23, 2019·Microbiology Spectrum·Martin VestergaardHanne Ingmer

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