Transfer of useful variability of high grain iron and zinc from Aegilops kotschyi into wheat through seed irradiation approach

International Journal of Radiation Biology
Shailender Kumar VermaHarcharan Singh Dhaliwal

Abstract

To transfer the 2S chromosomal fragment(s) of Aegilops kotschyi (2S(k)) into the bread wheat genome which could lead to the biofortification of wheat with high grain iron and zinc content. Wheat-Ae. kotschyi 2A/2S(k) substitution lines with high grain iron and zinc content were used to transfer the gene/loci for high grain Fe and Zn content into wheat using seed irradiation approach. Bread wheat plants derived from 40 krad-irradiated seeds showed the presence of univalents and multivalents during meiotic metaphase-I. Genomic in situ hybridization analysis of seed irradiation hybrid F2 seedlings showed several terminal and interstitial signals indicated the introgression of Ae. kotschyi chromosome segments. This proves the efficacy of seed radiation hybrid approach in gene transfer experiments. All the radiation-treated hybrid plants with high grain Fe and Zn content were analyzed with wheat group 2 chromosome-specific polymorphic simple sequence repeat markers to identify the introgression of small alien chromosome fragment(s). Radiation-induced hybrids showed more than 65% increase in grain iron and 54% increase in Zn contents with better harvest index than the elite wheat cultivar WL711 indicating effective and compensating t...Continue Reading

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Citations

Jan 21, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ankita SharmaShailender Kumar Verma
Feb 24, 2017·Journal of Inorganic Biochemistry·Shailender Kumar VermaYusuf Akhter
Apr 8, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Pushpendra Kumar GuptaRahul Kumar
Oct 26, 2016·Journal of Food Science and Technology·Jasmeet SinghH S Dhaliwal
May 31, 2019·Frontiers in Plant Science·Masahiro Kishii
Nov 3, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P K GuptaRahul Kumar

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