PMID: 6986171Jan 22, 1980Paper

Transfer ribonucleic acid guanine transglycosylase isolated from rat liver

Biochemistry
N Shindo-OkadaS Nishimura

Abstract

Transfer ribonucleic acid (tRNA) guanine transglycosylase (guanine insertion enzyme) was isolated from rat liver and extensively purified. The enzyme catalyzes an exchange of queuine (the base of queuosine, Q) as well as its precursors and guanine for guanine originally located in the first position of the anticodon of "undermodified" tRNATyr, tRNAHis, tRNAAsn, and tRNAAsp from an Escherichia coli mutant or rat ascites hepatoma cells. This is in contrast to the previous observation that E. coli tRNA-guanine transglycosylase catalyzes the exchange of queuine precursors, such as 7-(aminoethyl)-7-deazaguanine and 7-cyano-7-deazaguanine, but not of queuine itself [Okada, N., Noguchi, S. Kasai, H., Shindo-Okada, N., Ohgi, T., Goto, T., & Nishimura, S. (1979) J. Biol. Chem. 254, 3067-3073]. The Km value for queuine of the rat liver enzyme is 9.2 X 10(-7) M, much lower than the values for the bases of queuosine precursors or guanine. Thus, the actual substrate for tRNA-guanine transglycosylase in queuosine biosynthesis in vivo in rat liver may not be 7-(aminomethyl)-7-deazaguanine, which is thought to be an actual substrate guanine, the E. coli system. Queuine or some queuine derivative may be the actual substrate for the tRNA-guanine...Continue Reading

References

Dec 1, 1978·Nucleic Acids Research·R D McKinnonB N White
Oct 1, 1976·Nucleic Acids Research·W R Farkas, D Chernoff
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·N OkadaS Nishimura
Feb 1, 1977·European Journal of Biochemistry·M SilberklangU L Rajbhandary
Aug 4, 1976·Journal of the American Chemical Society·H KasaiS Nishimura

❮ Previous
Next ❯

Citations

Jan 1, 1989·Cancer Chemotherapy and Pharmacology·D S GibboneyR W Trewyn
Jan 9, 2007·Journal of Biosciences·Susumu Nishimura, Kimitsuna Watanabe
Jan 15, 1982·Biochemical and Biophysical Research Communications·M S Elliott, R W Trewyn
Feb 24, 1984·Biochimica Et Biophysica Acta·W R FarkasJ R Katze
Jul 7, 1992·Biochimica Et Biophysica Acta·U GündüzJ R Katze
Jan 31, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Annie CostaJean Desgrès
Jun 11, 2002·Journal of Molecular Biology·Ryuichiro IshitaniShigeyuki Yokoyama
Apr 17, 2003·Bioorganic Chemistry·Dirk Iwata-Reuyl
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B T FrenchR W Trewyn
May 6, 2009·The Journal of Biological Chemistry·Coilin BolandVincent P Kelly
Apr 11, 1993·Nucleic Acids Research·A Janke, S Pääbo
Jul 11, 1995·Nucleic Acids Research·R C MorrisM S Elliott
Oct 22, 2008·Journal of Bacteriology·Gabriella PhillipsValérie de Crécy-Lagard
Apr 18, 2015·Nutrients·Claire FergusVincent P Kelly
Mar 3, 2012·Bioorganic Chemistry·Reid M McCarty, Vahe Bandarian
Jun 30, 2012·Angewandte Chemie·Thomas CarellMirko Wagner
Oct 11, 2001·Molecular Genetics and Metabolism·R C Morris, M S Elliott
Sep 16, 1980·Biochemical and Biophysical Research Communications·J R Katze, W T Beck
Jun 1, 1988·Analytical Biochemistry·G MuralidharR W Trewyn
Sep 15, 1981·Toxicology and Applied Pharmacology·K B JacobsonN T Christie
Jun 7, 2011·Biochemical and Biophysical Research Communications·C Eric ThomasGeorge A Garcia
Nov 16, 1982·Biochemical and Biophysical Research Communications·U Gündüz, J R Katze
Dec 4, 2012·Protein Expression and Purification·Yuichiro NomuraTakashi Yokogawa
Nov 21, 2009·Bioscience Reports·Manjula Vinayak, Chandramani Pathak
Apr 15, 1983·Analytical Biochemistry·J P Reyniers, W R Farkas
Aug 31, 1990·Biochemical and Biophysical Research Communications·M S Elliott, D L Crane
Aug 31, 1990·Biochemical and Biophysical Research Communications·M S Elliott, D L Crane
Sep 21, 2005·Molecular Biology Reports·C Pathak, Manjula Vinayak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.