PMID: 4103Mar 17, 1976

Transfer RNA methyltransferase activity in paramecium aurelia

Biochimica Et Biophysica Acta
R A MakiD J Cummings

Abstract

The tRNA methyltransferases from Paramecium aurelia were investigated. The effects of varying the Mg2+ and NH4+ concentrations, pH, and temperature on the methylation of Escherichia coli B tRNA using extracts from P. aurelia were determined. Optimum tRNA methyltransferase activity was observed at pH 7.8 and 37 degrees C. The Mg2+ optimum occurred at 0.66 mM in the absence of NH4+ while the NH4+ optimum occurred at 100 mM in the absence of Mg2+. Analysis of the bases methylated in (E. coli B) tRNA by extracts of P. aurelia showed the presence of 1-methyladenine, 1-methylguanine, N2-methylguanine, N2,N2-dimethylguanine and methylated pyrimidine nucleotides. In comparison, an analysis of the in vivo methylation of tRNA from P. aurelia showed the presence of 1-methyladenine, 6-methyladenine, 6,6-dimethyladenine, 1-methylguanine, N2-methylguanine, N2,N2-dimethylguanine, 7-methylguanine, and methylated pyrimidine nucleotides. The pattern of methylation of tRNA in P. aurelia is similar to that observed in other eukaryotes.

Citations

Jan 19, 2021·Journal of Oral and Maxillofacial Pathology : JOMFP·Rizwan HamidMuzafar Bhat

Related Concepts

Ammonia
Metazoa
Guanine
Hydrogen-Ion Concentration
Magnesium
Paramecium
Transfer RNA
TRNA Methyltransferases
Gumbaral

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