Transferrin in after-cataract and as a survival factor for lens epithelium

Experimental Eye Research
M G DavidsonM C McGahan

Abstract

The Fe-transport protein, transferrin (Tf), is synthesized and secreted by whole lenses and cultured lens epithelial cells. Because of Tf's central role in cell growth and proliferation, its participation in lens cell proliferation following cataract extraction was explored using a rabbit model of after-cataract. Varying amounts of the central anterior lens capsule were removed (0, 35, or 80%) following extraction of the lens. The Tf content of and secretion by after-cataract lens capsular sacs containing regenerated lens tissue was determined ex vivo at 0, 3, 5, 7 and 9 weeks post-surgery. In all cases Tf content of and secretion by the lens sacs was higher than that of their contralateral controls (whole lenses). Tf secretion was up to 5-fold higher and metabolic labeling studies indicated secretion of newly synthesized Tf. The sacs contained up to 10 times the concentration of Tf as the control lenses. Human lens after-cataract capsular bags also secreted Tf. The function of Tf as a survival factor was tested on cultured lens epithelial cells. Cells cultured in serum-free medium had a survival rate of only 20-34% if the medium was changed each day. If the medium was never changed during this period, the survival rate was 43-...Continue Reading

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Citations

Aug 26, 1999·The British Journal of Ophthalmology·N OhguroY Tano
Mar 17, 2010·Experimental Eye Research·Sixto García-Castiñeiras
May 29, 2010·Journal of Pediatric Ophthalmology and Strabismus·Inbal MichaelShulamit Levenberg
May 6, 2015·Experimental Eye Research·Ian Michael Wormstone, Julie Ann Eldred
Feb 1, 2006·Environmental Toxicology and Pharmacology·I Michael WormstoneGeorge Duncan
May 17, 2002·Experimental Eye Research·I Michael Wormstone
Jan 15, 1999·Journal of Cataract and Refractive Surgery·O Nishi
Jul 1, 1999·Current Opinion in Ophthalmology·J Emery
Jan 10, 2001·Current Opinion in Ophthalmology·E Bertelmann, C Kojetinsky
Sep 2, 2020·Journal of Cellular Physiology·Bi Ning ZhangYusen Huang
Jan 11, 2021·Experimental Eye Research·Arjun JaitliLiping Tang
Mar 17, 2021·Free Radical Biology & Medicine·Zongbo WeiXingjun Fan
Jan 7, 2000·Biochemical and Biophysical Research Communications·D P SinghT Shinohara
Sep 26, 2020·Progress in Retinal and Eye Research·I M WormstoneJ A Eldred

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