Transferrin receptor induction in Toxoplasma gondii-infected HFF is associated with increased iron-responsive protein 1 activity and is mediated by secreted factors

Parasitology Research
Markus GailWolfgang Bohne

Abstract

Infection with the apicomplexan parasite Toxoplasma gondii results in a significant alteration of the host-cell transcriptional profile. We have previously shown that the transferrin receptor (TfR) is specifically up-regulated in T. gondii-infected human fibroblasts but not in host cells infected with the bacterial pathogens Salmonella Typhimurium and Chlamydia trachomatis. In this report, we describe the prerequisites and physiological conditions that are associated with this pathogen-specific gene induction. Band-shift assays revealed that T. gondii infection resulted in increased activity in the iron response protein IRP1, which, in this state, stabilizes TfR mRNA from degradation. Although T. gondii depends on host-cell iron as demonstrated by sensitivity to deferoxamine, a parasite-induced iron starvation is not responsible for TfR up-regulation. The increased iron availability due to treatment with holotransferrin and FeNTA did not prevent TfR induction nor was the transferrin-independent iron-transporter NRAMP2 up-regulated in infected host cells. In addition, inhibition of parasite replication by drug treatment did not prevent TfR up-regulation. Instead, TfR induction was sensitive to cycloheximide and could be induced ...Continue Reading

References

Aug 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·B J Luft, J S Remington
Jun 1, 1994·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S Y Wong, J S Remington
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·J C SchwabK A Joiner
Oct 1, 1995·Trends in Microbiology·F Garcia-del Portillo, B B Finlay
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M W Hentze, L C Kühn
Jun 1, 1997·European Journal of Immunology·H G FischerU Hadding
Jan 1, 1997·Annual Review of Microbiology·A P Sinai, K A Joiner
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·K Pantopoulos, M W Hentze
Mar 20, 1999·Infection and Immunity·C F DenneyS L Reed
May 5, 1999·Current Opinion in Chemical Biology·P AisenE A Leibold
Jun 22, 1999·Infection and Immunity·D Howe, L P Mallavia
Oct 16, 1999·The American Journal of the Medical Sciences·D J Haile
Mar 14, 2000·Current Opinion in Immunology·I D Manger, D A Relman
Apr 11, 2001·The Journal of Biological Chemistry·I J BladerJ C Boothroyd

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Citations

Mar 11, 2008·Cellular and Molecular Life Sciences : CMLS·J Laliberté, V B Carruthers
Jun 10, 2011·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Changlu LiNing Li
May 16, 2006·Immunobiology·Sek Tong OngJeak Ling Ding
Jun 23, 2015·Cellular Microbiology·Kevin Cheeseman, Jonathan B Weitzman
Feb 7, 2012·Experimental Parasitology·Thaís Alves da Costa-SilvaVera Lucia Pereira-Chioccola
Nov 24, 2018·F1000Research·Martin Blume, Frank Seeber

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