Transformation and analysis of tobacco plant var Petit havana with T-urf13 gene under anther-specific TA29 promoter.

3 Biotech
V ArunGeorge Thomas

Abstract

T-urf13, a well-documented cms-associated gene from maize, has been shown to render methomyl sensitivity to heterologous systems like rice, yeast and bacteria when expressed constitutively. Since these transgenic plants were fertile, it was hypothesized that T-urf13 gene if expressed in anthers may result in male sterility that could be used for hybrid seed production. Hence, this work was aimed at analysing whether T-urf13 gene when expressed in anthers can result in male sterile plants or requires methomyl treatment to cause male sterility (controllable). This is the first report of transformation of tobacco with T-urf13 gene under anther-specific promoter (TA29) with or without mitochondrial targeting sequence. Most of the transgenic plants obtained were fertile; this was surprising as many male sterile plants were expected as T-urf13 gene is a cms associated gene. Our results suggest that it may not be possible to obtain male sterility by expressing URF13 in the anther by itself or by methomyl application.

References

Oct 1, 1991·Molecular & General Genetics : MGG·J M von AllmenD M Lonsdale
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C J BraunC S Levings
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·F ChaumontM Boutry
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K L Korth, C S Levings
Nov 8, 1996·Science·M R Emmert-BuckL A Liotta
Oct 1, 1993·The Plant Cell·C. S. Levings
Mar 27, 2003·Genetica·Françoise BudarRosine De Paepe
May 15, 2003·Plant Physiology·Nancy M KerkTimothy M Nelson
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B G Forde, C J Leaver
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R E DeweyC S Levings

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Datasets Mentioned

BETA
X52283

Methods Mentioned

BETA
transgenic
PCR
X-ray
dissection

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