Transformation and regeneration of the self-incompatible species Nicotiana alata Link & Otto

Plant Molecular Biology
P. R. Ebert, A E Clarke

Abstract

A transformation and regeneration system has been developed for Nicotiana alata, a plant which is being intensively studied as a model of gametophytic self-incompatibility. Plantlets can be regenerated efficiently from seedling hypocotyls. Kanamycin-resistant, transformed plants have been obtained by cocultivation of regenerating hypocotyls with Agrobacterium tumefaciens strain LBA4404 containing a binary vector. The transformation frequency was low with less than 1% of tissue explants regenerating transformed plants. The transformed plants contained from one to three copies of the introduced DNA. In most cases, the kanamycin resistance phenotype was transmitted to the offspring as a normal Mendelian factor. In one unusual case, none of the offspring inherited the kanamycin resistance of the transformed maternal parent. This plant may have been chimeric or the kanamycin resistance gene may have been inactivated.

References

Feb 1, 1978·Plasmid·D SciakyM D Chilton
Jan 1, 1988·Annual Review of Cell Biology·E C CornishA E Clarke
Mar 1, 1980·Journal of Bacteriology·F F White, E W Nester
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P R EbertG An
Sep 1, 1988·Plant Molecular Biology·X C ZhanA Kerr
May 1, 1977·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K Sree RamuluM Devreux

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