PMID: 6777630Jan 1, 1980

Transformation and transduction of Bacillus subtilis strains with the Bsu R restriction-modification system by means of modified and unmodified DNA of pUB110 plasmid

Molecular & General Genetics : MGG
A A ProzorovN N Surikov

Abstract

During transformation of B. subtilis cells with the Bsu R restriction-modification system by means of pUB110 plasmid restriction and modification of the plasmid DNA occurs. The effect of restriction on the transformation frequency is relatively weak, bringing about 20-fold decrease only. When using cells of a modifying recipient, the frequency of AR9 phage-mediated transduction of unmodified plasmid DNA is also relatively little decreased. The frequency of transduction by chromosomal markers, under the same conditions, falls much lower.

References

Dec 1, 1978·The Journal of General Virology·E FerrariG Mazza
Sep 1, 1979·Molecular & General Genetics : MGG·T Tanaka
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T Gryczan, D Dubnau
Jan 1, 1974·Molecular & General Genetics : MGG·T A TrautnerC Anagnostopoulos

Related Concepts

Natto Bacteria
Bacteriophages
DNA, Bacterial
DNA, Viral
Genetic Markers
Episomes
Transduction, Genetic
Transformation, Genetic

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