PMID: 6159984Oct 1, 1980Paper

Transformation by Rous sarcoma virus: a cellular substrate for transformation-specific protein phosphorylation contains phosphotyrosine

Cell
K RadkeG S Martin

Abstract

Transformation of chicken embryo fibroblasts by Rous sarcoma virus (RSV) is caused by a single viral gene, src, which encodes a phosphoprotein, pp60src, with the enzymatic activity of a protein kinase. The relative abundance of a 36,000 molecular weight (36K) phosphorylated polypeptide which can be detected by two-dimensional electrophoresis of 32P-labeled phosphoproteins is greatly increased in RSV-transformed fibroblasts. We have reported previously that phosphorylation of the 36K polypeptide is an early event in the process of transformation and that protein synthesis is not required for its appearance. Here we identify a nonphosphorylated 36K polypeptide, present in both uninfected and transformed cells, which is homologous to the 36K phosphoprotein as judged by limited proteolysis and by tryptic peptide mapping. We conclude that the 36K phosphoprotein is generated by phosphorylation of this 36K polypeptide. It has recently been shown that pp60src phosphorylates tyrosine residues in vitro: phosphotyrosine and also phosphoserine are present in the 36K phosphoprotein isolated from RSV-transformed cells. On the basis of these results we propose that the 36K polypeptide present in chicken fibroblasts is a substrate for the prot...Continue Reading

Citations

Jan 1, 1986·Molecular Biology Reports·M Sluyser, O H Destrée
May 1, 1994·In Vitro Cellular & Developmental Biology. Animal·A S MaA Tranvan
Jan 1, 1985·Pharmacology & Therapeutics·D R MakowskiS M Astrin
Feb 1, 1988·Virus Research·L H Wang, H Hanafusa
Jan 1, 1984·Biochimica Et Biophysica Acta·M H Cobb, O M Rosen
Nov 25, 1987·Biochimica Et Biophysica Acta·C H HeldinB Westermark
Jun 7, 1996·Biochimica Et Biophysica Acta·M T Brown, J A Cooper
Jan 20, 2000·Trends in Cardiovascular Medicine·K A Hajjar, S Krishnan
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A LaszloM J Bissell
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B GallisD L Brautigan
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B M SeftonW C Raschke
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Y S Cheng, L B Chen
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S Decker
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H RübsamenE Eigenbrodt
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·V Rotter
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D ShallowayP Yaciuk
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J R Glenney, B F Tack
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y FukamiT Kanehisa
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Glenney
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·G ScholzH Hanafusa
May 25, 2002·Journal of Biomolecular Structure & Dynamics·Alexei Kurakin, Dale Bredesen
Dec 1, 1988·The Journal of Cell Biology·C Grandori, H Hanafusa
May 29, 1998·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·T Hunter
Jul 1, 1982·European Journal of Biochemistry·G GaconS Fischer
Feb 15, 1983·European Journal of Biochemistry·A WeberA Kahn
Oct 15, 1988·European Journal of Biochemistry·S E MossM J Crumpton
May 23, 1991·European Journal of Biochemistry·H C Edwards, M J Crumpton

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.