PMID: 108261May 1, 1979

Transformation-derived Neisseria gonorrhoeae plasmids with altered structure and function

Journal of Bacteriology
T E SoxP F Sparling


Plasmid deoxyribonucleic acid from Neisseria gonorrhoeae containing a 7.1-kilobase (kb) (4.7-megadalton) penicillinase (Pcr) plasmid transformed homogenic gonococci to penicillinase production at a low frequency. About 25% of the penicillinase-producing gonococcal transformants contained Pcr plasmids which were either larger or smaller than the 7.1 kb donor plasmid; these Pcr plasmids varied in size from 3.45 to 42 kb. Some of these altered plasmids differed from the donor plasmid in stability or in frequency of mobilization by a 36-kb (24-megadalton) conjugative plasmid. A restriction endonuclease cleavage map of the 7.1-kilobase Pcr plasmid and several of the smaller deleted plasmids was constructed. The most common size of altered Pcr plasmid was 5.1 kb (3.4 megadaltons). A Pcr plasmid isolated from a gonococcus in London, England, was identical with these 5.1-kb transformant plasmids in both size and restriction endonuclease cleavage profiles, suggesting that the 5.1-kb Pcr plasmid could have arisen from a 7.1-kb Pcr plasmid by a transformation-associated deletion in nature.


Feb 1, 1987·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·R B Aalen, W B Gundersen
Jan 5, 2006·Molecular Microbiology·Holly L Hamilton, Joseph P Dillard
May 1, 1988·Molecular & General Genetics : MGG·J C PiffarettiJ Frey
Jul 2, 2014·Clinical Microbiology Reviews·M Unemo, William M Shafer

Related Concepts

Bacterial Conjugation
DNA Restriction Enzymes
DNA, Bacterial
Alkalescens-Dispar Group
Neisseria gonorrhoeae
Transformation, Genetic

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