Transformation fingerprint: induced STAT3-C, v-Src and Ha-Ras cause small initial changes but similar established profiles in mRNA

Oncogene
Keren PazJames E Darnell

Abstract

Induced transformation of mouse fibroblasts was carried out by releasing tetracycline-repressed expression of an oncogenic mutant of STAT3, STAT3-C, or of v-Src or Ha-Ras. At 15 days after derepression of each oncogene, DNA microarrays showed elevation (>3-fold) of a similar group of approximately 25 mRNAs compared to untransformed cells. RT-PCR confirmed a number of these mRNA elevations. RNA samples were then analysed at intervals during the first 24 h after doxycycline removal to determine the time of early changes. Extensive changes were not observed by array analysis, except in v-Src-expressing cells where about 10 mRNAs were elevated threefold or more. However, RT-PCR did uncover changes in each derepressed cell type that included some of the changes observed after the 15-day transformation period. In addition, STAT3-C target genes such as BclXI and cyclin D1, which were not observed on array analysis, were elevated by RT-PCR analysis. We conclude, therefore, that early after oncogene induction, transcriptional changes, including those initiated by STAT3-C, may occur only in scarce mRNA and/or to a limited extent. However, with additional time and probably additional cell division, a new epigenetic state is established th...Continue Reading

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Citations

Oct 9, 2007·Journal of Cancer Research and Clinical Oncology·Mikio TomidaMasafumi Kurosumi
Aug 6, 2009·PloS One·Hong-Zhen YangZhuo-Wei Hu
Dec 10, 2015·Molecular Carcinogenesis·Jaya Srivastava, John DiGiovanni
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Dec 21, 2012·Journal of Cellular Physiology·Tiziana DeangelisRenato Baserga
Aug 6, 2005·Biochemical and Biophysical Research Communications·Fu-Chuan HsiehJiayuh Lin
Dec 11, 2007·The Journal of Biological Chemistry·Marylynn SnyderJ Jillian Zhang
Mar 4, 2006·Science·Yingfeng QiaoPhilip A Cole

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