PMID: 16592896Oct 1, 1980Paper

Transformation in Agmenellum quadruplicatum

Proceedings of the National Academy of Sciences of the United States of America
S E Stevens, R D Porter

Abstract

A DNA-mediated transformation system for the blue-green alga Agmenellum quadruplicatum, strain PR-6, is described and characterized for DNA concentration dependence, dependence on time of exposure to DNA, phenotypic expression, sensitivity to various enzymes, and competence. The stability of the transformants has been investigated, and genetic backcross and selfing experiments have been performed. This system fulfills all of the criteria established for the well-characterized transformation systems in heterotrophic bacteria and demonstrates significant similarities to at least one of these systems for all characteristics examined. The efficiency of transformation is high. This system fills a need for a well-characterized genetic system in an oxygen-evolving photoautotroph. We have used it to transform a strain with a mutational lesion in assimilatory nitrogen metabolism to a wild-type genotype.

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Citations

Jan 4, 1994·Biochimica Et Biophysica Acta·R PalmenK J Hellingwerf
Apr 15, 1994·Journal of Biotechnology·K SodeT Matsunaga
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Jul 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Julia A MarescaDonald A Bryant
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May 29, 2012·Biotechnology Advances·Song QinPeng Jiang
Mar 19, 2013·Bioresource Technology·Matthew R MelnickiAlexander S Beliaev
Aug 26, 2014·Frontiers in Bioengineering and Biotechnology·Anne M Ruffing
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Mar 14, 2017·Biotechnology for Biofuels·Joachim KopkaAlexandra Friedrich
Feb 15, 2015·Molecular & Cellular Proteomics : MCP·Qian XiongJin-dong Zhao
Feb 8, 2011·Journal of Bacteriology·Richard M AlveyDonald A Bryant
Oct 11, 2015·Applied and Environmental Microbiology·Bo WangDeirdre R Meldrum

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