Transformation in Bacillus subtilis: purification and partial characterization of a membrane-bound DNA-binding protein.

Journal of Bacteriology
H SmithG Venema

Abstract

In DNA binding-deficient mutants of Bacillus subtilis a competence-specific protein with a subunit molecular weight of 18,000 was absent. The native protein containing this subunit was purified from B. subtilis membranes by chromatography on hydroxyapatite, DEAE-cellulose, and Sephacryl S-200. This protein appeared to be complexed with a second protein of slightly lower molecular weight (17,000) and a different isoelectric point. The native protein complex (apparent molecular weight, 75,000) contained approximately equal amounts of the two polypeptides and showed a strong DNA-binding activity. Incubation of the complex with plasmid and bacteriophage DNA revealed nuclease activity, specifically directed toward double-stranded DNA. Predominantly single-stranded nicks and a limited number of double-stranded breaks were introduced in the presence of Mg2+ ions. In the presence of Mn2+ ions the complex produced low-molecular-weight breakdown products from the DNA.

References

Oct 1, 1979·Molecular & General Genetics : MGG·B BehrensA T Ganesan
Jan 1, 1979·Advances in Microbial Physiology·G Venema
Apr 11, 1973·Biochimica Et Biophysica Acta·D A Morrison, W R Guild
Jan 1, 1967·Molecular & General Genetics : MGG·N BiswalT A Trautner
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock

❮ Previous
Next ❯

Citations

Sep 1, 1986·Molecular & General Genetics : MGG·B VosmanG Venema
May 22, 2001·Molecular Microbiology·R ProvvediD Dubnau
Oct 7, 2015·Bioprocess and Biosystems Engineering·Sushma ChityalaReddy Shetty Prakasham
Aug 1, 1986·Molecular & General Genetics : MGG·H Daniell, B A McFadden
Jan 30, 2008·The Journal of Biological Chemistry·Jonathan C GauntlettGregory M Cook
Aug 23, 2019·Annual Review of Genetics·David Dubnau, Melanie Blokesch
Oct 1, 1989·Journal of Bacteriology·M AlbanoD A Dubnau
Sep 1, 1991·Microbiological Reviews·D Dubnau

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.