Transformation in Streptococcus pneumoniae: formation of eclipse complex in a coiA mutant implicates CoiA in genetic recombination

Molecular Microbiology
Bhushan V Desai, Donald A Morrison

Abstract

CoiA is a transient protein expressed specifically during competence and required for genetic transformation in Streptococcus pneumoniae, but not for DNA uptake. It is widely conserved among Gram-positive bacteria but its function is unknown. Here we report that although the rate of DNA uptake was not affected in a coiA mutant, the internalized donor DNA did not recombine into the host chromosome to form a physical and genetic heteroduplex. Instead, DNA taken up by a coiA mutant accumulated in the form of a single-stranded (ss) DNA-protein complex indistinguishable from the eclipse complex formed as a recombination intermediate in wild-type competent cells. Internalized donor DNA in a dprA mutant did not accumulate either as ss DNA or as an eclipse complex. Together, these results establish that a coiA mutant exhibits a phenotype different from that of dprA or recA mutants, and that CoiA functions at a later step in promoting recombination during genetic transformation in Streptococcus pneumoniae.

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Citations

Jul 3, 2007·Journal of Bacteriology·Donald A MorrisonJean-Pierre Claverys
Nov 13, 2007·Research in Microbiology·Ola JohnsborgLeiv Sigve Håvarstein
Jul 17, 2007·Molecular Microbiology·Naomi KramerDavid Dubnau
Aug 27, 2014·Molecular Microbiology·Annette FagerlundLeiv Sigve Håvarstein
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Oct 11, 2012·Critical Reviews in Biochemistry and Molecular Biology·Dawit KidaneJuan C Alonso
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Related Concepts

DprA protein, bacteria
CoiA protein, Streptococcus pneumoniae
Bacterial Proteins
DNA, Single-Stranded
Hydroxyphenylazouracil
Cell Surface Proteins
Heteroduplex DNA
Recombination, Genetic
Streptococcus pneumoniae
Transformation, Bacterial

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