PMID: 6412037Jan 1, 1983Paper

Transformation in the cyanobacterium Synechococcus R2: improvement of efficiency; role of the pUH24 plasmid

Molecular & General Genetics : MGG
Franck ChauvatF Joset-Espardellier

Abstract

Systematic studies of the parameters influencing transformation efficiencies in Synechococcus R2 and its spontaneous pUH24 plasmid-minus variant S60, have led to substantial increases in these frequencies (up to 70 times for R2 and 9 times for S60) compared to those obtained by using previously published procedures. Important factors are the physiological state of the host cells (competence occurs at the transition from first to second exponential phases of growth) and the conditions of expression of the transferred characters (they must be exposed to progressively increasing concentrations of the selective agent). Consistently lower (about 30 times) efficiencies were obtained with the S60 strain, the only difference from the wild-type R2 that could be detected. Physiological functions of the pUH24 plasmid are discussed. The interest of the S60 strain as a host in gene cloning work is discussed in the light of published work suggesting inter-vector recombination in R2.

References

Oct 1, 1978·Journal of Bacteriology·R D Simon
Jul 1, 1979·Plasmid·C A van den HondelG A van Arkel
Jun 7, 1974·Archiv für Mikrobiologie·K G Orkwiszewski, A R Kaney
Nov 1, 1973·Journal of Bacteriology·P GuerryS Falkow
Jan 1, 1973·Molecular & General Genetics : MGG·M Herdman
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C A van den HondelG A van Arkel

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Citations

Aug 1, 1986·Molecular & General Genetics : MGG·H Daniell, B A McFadden
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H DaniellB A McFadden
Jan 1, 1986·Critical Reviews in Microbiology·R D Porter
Sep 26, 2019·New Zealand Journal of Botany·Susan S Golden
Apr 1, 1984·Journal of Bacteriology·S S Golden, L A Sherman
Apr 1, 1990·Journal of Bacteriology·E EssichR D Porter
Oct 1, 1987·Journal of Bacteriology·S M Gendel
Sep 1, 1994·Microbiological Reviews·M G Lorenz, W Wackernagel

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