Transformation Kinetics of Chlorinated Ethenes by Methylosinus trichosporium OB3b and Detection of Unstable Epoxides by On-Line Gas Chromatography.

Applied and Environmental Microbiology
V J Van HylckamaD B Janssen

Abstract

A rapid and accurate method for the determination of transformation kinetics of volatile organic substrates was developed. Concentrations were monitored by on-line gas chromatographic analysis of the headspace of well-mixed incubation mixtures. With this method, the kinetics of transformation of a number of C(inf1) and C(inf2) halogenated alkanes and alkenes by Methylosinus trichosporium OB3b expressing particulate methane monooxygenase or soluble methane monooxygenase (sMMO) were studied. Apparent specific first-order rate constants for cells expressing sMMO decreased in the order of dichloromethane, vinyl chloride, cis-1,2-dichloroethene, trans-1,2-dichloroethene, 1,1-dichloroethene, trichloroethene, chloroform, and 1,2-dichloroethane. During the degradation of trichloroethene, cis-1,2-dichloroethene, trans-1,2-dichloroethene, and vinyl chloride, the formation of the corresponding epoxides was observed. The epoxide of vinyl chloride and the epoxide of trichloroethene, which temporarily accumulated in the medium, were chemically degraded according to first-order kinetics, with half-lives of 78 and 21 s, respectively. Cells expressing sMMO actively degraded the epoxide of cis-1,2-dichloroethene but not the epoxide of trans-1,2-...Continue Reading

References

Jan 1, 1979·Biochemical Pharmacology·D HenschlerM Metzler
Nov 17, 1975·Biochemical and Biophysical Research Communications·A BarbinH Bartsch
Apr 1, 1992·Applied and Environmental Microbiology·S Hartmans, J A De Bont
Jan 1, 1990·Archives of Microbiology·J EwersH J Knackmuss
Dec 1, 1989·Applied and Environmental Microbiology·H C TsienL P Waclett
Nov 1, 1989·Applied and Environmental Microbiology·L P WackettR S Hanson
Mar 15, 1989·Biochemical and Biophysical Research Communications·D ArcieroA B Hooper
Jul 1, 1988·Applied and Environmental Microbiology·L P Wackett, D T Gibson
May 1, 1987·Applied and Environmental Microbiology·M J NelsonP H Pritchard
May 1, 1985·Journal of Bacteriology·D Kohler-Staub, T Leisinger
Mar 1, 1985·Applied and Environmental Microbiology·D B JanssenB Witholt
Aug 1, 1995·Applied and Environmental Microbiology·M W ReijS Hartmans
Jul 1, 1993·Applied and Environmental Microbiology·A J van den WijngaardD B Janssen
Sep 20, 1993·Biotechnology and Bioengineering·D E FennellW J Jewell
Aug 1, 1995·Environmental Science & Technology·M F TschantzG S Sayler

❮ Previous
Next ❯

Citations

Apr 19, 2011·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Jian B LiuKiminori Itoh
Jun 1, 2002·Applied and Environmental Microbiology·Nicholas V ColemanJim C Spain
Aug 10, 2010·Applied and Environmental Microbiology·Huifeng ShanDavid L Freedman
Feb 12, 2010·FEMS Microbiology Reviews·Timothy E MattesNicholas V Coleman
May 27, 2004·Ground Water·Young KimLewis Semprini
Mar 9, 2005·Applied Microbiology and Biotechnology·Kimberly H HalseyDaniel J Arp
Oct 25, 2012·Applied Microbiology and Biotechnology·M CappellettiS Fedi
Jan 4, 2018·Applied Microbiology and Biotechnology·Anne E TaylorLewis Semprini
Jul 27, 2001·Applied and Environmental Microbiology·I J DinklaD B Janssen
Jan 15, 1999·Critical Reviews in Microbiology·J P SullivanH A Chase
Apr 25, 2001·Applied and Environmental Microbiology·C M YeagerD J Arp
Feb 18, 2004·Journal of Environmental Quality·Charlotte ScheutzPeter Kjeldsen
Jun 29, 2010·Applied Microbiology and Biotechnology·Anne E TaylorLewis Semprini
Sep 6, 2008·FEMS Microbiology Letters·Sukhwan Yoon, Jeremy D Semrau
May 2, 2000·Applied and Environmental Microbiology·K McClayR J Steffan
Mar 20, 2010·FEMS Microbiology Reviews·Jeremy D SemrauSukhwan Yoon
Jan 9, 2019·Frontiers in Microbiology·Siavash AtashgahiDetmer Sipkema
Apr 13, 2017·Environmental Science and Pollution Research International·Yi LiangTimothy E Mattes
Nov 27, 2008·Environmental Science & Technology·Gwenaël ImfeldHans H Richnow
Jun 1, 1997·Current Opinion in Biotechnology·L Semprini
Feb 13, 2001·Journal of Biotechnology·J E van Hylckama Vlieg, D B Janssen
Dec 14, 2005·Applied and Environmental Microbiology·A E MarsD B Janssen
Nov 15, 1997·Applied and Environmental Microbiology·K S SmithM E Lidstrom
Mar 15, 2006·Applied and Environmental Microbiology·V J Van HylckamaD B Janssen
Nov 3, 2010·Environmental Science & Technology·Yang Oh Jin, Timothy E Mattes
Feb 27, 2016·Environmental Science & Technology·Margaret FindlayTimothy E Mattes

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.