Transformation of Amorphous Polyphosphate Nanoparticles into Coacervate Complexes: An Approach for the Encapsulation of Mesenchymal Stem Cells

Small
Werner E G MüllerXiaohong Wang

Abstract

Inorganic polyphosphate [polyP] has proven to be a promising physiological biopolymer for potential use in regenerative medicine because of its morphogenetic activity and function as an extracellular energy-donating system. Amorphous Ca2+ -polyP nanoparticles [Ca-polyP-NPs] are characterized by a high zeta potential with -34 mV (at pH 7.4). This should contribute to the stability of suspensions of the spherical nanoparticles (radius 94 nm), but make them less biocompatible. The zeta potential decreases to near zero after exposure of the Ca-polyP-NPs to protein/peptide-containing serum or medium plus serum. Electron microscopy analysis reveals that the particles rapidly change into a coacervate phase. Those mats are amorphous, but less stable than the likewise amorphous Ca-polyP-NPs and are morphogenetically active. Mesenchymal stem cells grown onto the polyP coacervate show enhanced growth/proliferation and become embedded in the coacervate. These results suggest that the Ca-polyP coacervate, formed from Ca-polyP-NPs in the presence of protein, can act as an adaptable framework that mimics a niche and provides metabolic energy in bone/cartilage engineering.

References

Jun 29, 2000·Annual Review of Biochemistry·A KornbergD Ault-Riché
May 1, 1957·Journal of Cellular Physiology. Supplement·J T OVERBEEK, M J VOORN
Jan 26, 2005·FEMS Microbiology Letters·Srinivas Reddy PallerlaSiegfried M Schoberth
Mar 2, 2005·Nature Reviews. Microbiology·Roberto DocampoSilvia N J Moreno
Mar 24, 2007·Current Biology : CB·Damien CuvelierL Mahadevan
May 16, 2007·Current Biology : CB·James L McGrath
Aug 19, 2007·Biomaterials·Jindrich Kopecek
Aug 21, 2007·Biomaterials·Mark E FurthMark E Van Dyke
Apr 29, 2008·Biomaterials·David F Williams
May 29, 2008·The Journal of Physical Chemistry. a·Maurício A P SilvaLuiz Fernando C de Oliveira
Apr 7, 2009·Annual Review of Biochemistry·Narayana N RaoArthur Kornberg
Feb 4, 2010·The Journal of Biological Chemistry·Evgeny PavlovAndrey Y Abramov
Oct 26, 2011·Current Stem Cell Research & Therapy·Gopiga ThanabalasundaramWasim S Khan
Jan 15, 2014·Tissue Engineering. Part a·David F Williams
Apr 22, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Arash Momeni, Mark Joseph Filiaggi
Apr 29, 2014·BioMed Research International·Warangkana LohcharoenkalYon Rojanasakul
Jul 2, 2014·Tissue Engineering. Part a·Claudia LoebelMartin J Stoddart
Aug 21, 2014·Expert Opinion on Drug Delivery·Noah R Johnson, Yadong Wang
Sep 3, 2014·Journal of Cell Science·Peter StaporPeter Carmeliet
Nov 8, 2014·International Review of Cell and Molecular Biology·Xiaohong WangWerner E G Müller
Apr 23, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Werner E G MüllerXiaohong Wang
Apr 25, 2015·Journal of Cell Science·Werner E G MüllerXiaohong Wang
May 16, 2015·International Journal of Laboratory Hematology·R J TraversJ H Morrissey
May 20, 2015·Macromolecular Bioscience·Werner E G MüllerXiaohong Wang
Dec 3, 2016·European Cells & Materials·X WangW E Müller
Jul 9, 2017·Journal of Cell Science·Werner E G MüllerXiaohong Wang
Aug 2, 2017·Tissue Engineering. Part C, Methods·David F Williams
Aug 5, 2017·Cellular and Molecular Life Sciences : CMLS·Werner E G MüllerXiaohong Wang
Nov 18, 2017·Soft Matter·Mintai P HwangYadong Wang
Apr 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaohong WangWerner E G Müller
Feb 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Werner E G MüllerXiaohong Wang

❮ Previous
Next ❯

Citations

May 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Emad TolbaWerner E G Müller
Jan 30, 2020·Nature Reviews. Microbiology·M Fata Moradali, Bernd H A Rehm
Jun 20, 2019·International Journal of Molecular Sciences·Werner E G MüllerXiaohong Wang
Apr 9, 2021·Biochemistry. Biokhimii︠a︡·Tatiana V KulakovskayaMichail A Eldarov
Nov 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shunfeng WangWerner E G Müller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.