Transformation of an Exotic Yeast Species into a Platform Organism: A Case Study for Engineering Glycolipid Production in the Yeast Starmerella bombicola

Methods in Molecular Biology
Sofie LodensWim Soetaert

Abstract

In this chapter, a step-by-step approach on how to transform non-conventional yeasts or fungi into platform organisms is described. The non-conventional glycolipid producing yeast Starmerella bombicola (and in some cases also Pseudohyphozyma bogoriensis) is used as a case study. And more specifically how to engineer it toward production of new-to-nature glycolipids like bola sophorolipids. When starting genetic engineering efforts for non-lab strains, one should start at the very basis: identifying selection markers and possibly developing auxotrophic strains. Once this is done, the quest for the development of an optimal transformation method can be started. After optimization thereof, knock-out and knock-in strains can be generated based upon the specific strategy/aim. Sometimes this can lead to unexpected, but yet very interesting findings. To fully and efficiently expand the potential as a production platform of these yeast strains, a range of additional molecular tools are required. A well-equipped molecular toolbox should contain a set of characterized promotors, terminators, and defined genomic landing paths. The availability of an episomal system greatly facilitates engineering and screening efforts, but also offers the...Continue Reading

Citations

Apr 6, 2019·Applied Microbiology and Biotechnology·Lisa Van RenterghemWim Soetaert
Oct 11, 2019·FEMS Yeast Research·Marilyn De GraeveWim K G Soetaert
Apr 25, 2020·FEMS Yeast Research·Sofie LodensWim Soetaert
Jul 10, 2018·FEMS Yeast Research·Marilyn De GraeveWim Soetaert

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