Transformation of animal cells with human T-cell leukemia virus type II

Japanese Journal of Cancer Research : Gann
K MiyamotoT Akagi

Abstract

Human T-cell leukemia virus type II (HTLV-II) was tested for its ability to transform normal animal cells. The HTLV-II-infected human T-cell line, HTLV-IIA was lethally X-irradiated and cocultivated with normal leukocytes of rabbit and crab-eating monkey and spleen cells of hamster. The transformed cell lines, designated Ra-IIA, Si-IIA and Ham-IIA, were established. These cell lines were shown to be infected with HTLV-II by the polymerase chain reaction method combined with the digoxigenin-enzyme-linked immunosorbent assay method. These cell lines were examined for viral antigens by the indirect immunofluorescence method. Although the cytoplasma of over 90% of the cells of Si-IIA cell line was brilliantly stained, Ra-IIA and Ham-IIA cells were not stained. Electron microscopy of cells of the Si-IIA line revealed C-type virus particles in the extracellular spaces.

References

May 15, 1986·International Journal of Cancer. Journal International Du Cancer·T AkagiI Miyoshi
Aug 7, 1986·The New England Journal of Medicine·J D RosenblattI S Chen
Oct 15, 1984·International Journal of Cancer. Journal International Du Cancer·H HoshinoT Sugimura

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Citations

Nov 1, 1996·Critical Reviews in Oncology/hematology·T IwamasaK Miyamoto
Jan 1, 1992·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·K HayashiK Miyamoto
Feb 1, 1997·Japanese Journal of Cancer Research : Gann·H MachidaI Miyoshi
Jul 9, 1993·International Journal of Cancer. Journal International Du Cancer·N OharaT Akagi
May 1, 1992·Japanese Journal of Cancer Research : Gann·K MiyamotoK Kitajima

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