Transformation of Bacillus cereus vegetative cells by electroporation.

Applied and Environmental Microbiology
B H Belliveau, J T Trevors

Abstract

Transformation of untreated vegetative cells of Bacillus cereus 569 with plasmid pC194 (1.8 megadaltons) by high-voltage electroporation resulted in a maximum of 2 x 10(-5) transformants per viable cell. Transformation of a 130-megadalton plasmid occurred at a comparable frequency. The method was simple, rapid, and yielded transformant colonies in 14 to 24 h. Transformation was obtained with unpurified total plasmid DNA.

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Citations

Dec 1, 2009·Applied Microbiology and Biotechnology·Trond Erik Vee Aune, Finn Lillelund Aachmann
Apr 4, 2020·Molecular Biology Reports·Ibrahim Ilker Ozyigit
Feb 23, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Salwa Abdullah Sirajuddin, Shamala Sundram
Jan 24, 2020·NPJ Biofilms and Microbiomes·Joaquín Caro-AstorgaDiego Romero
Jan 1, 1990·Biology of Metals·B H Belliveau, J T Trevors
Jan 15, 2021·NPJ Biofilms and Microbiomes·Joaquín Caro-AstorgaDiego Romero
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·E SchnepfD H Dean

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