Transformation of cambial tissue in vivo provides an efficient means for induced somatic sector analysis and gene testing in stems of woody plant species

Functional Plant Biology : FPB
Kim S Van BeverenGerd Bossinger

Abstract

Large-scale functional analysis of genes and transgenes suspected to be involved in wood development in trees is hindered by long generation times, low transformation and regeneration efficiencies and difficulties with phenotypic assessment of traits, especially those that appear late in a tree's development. To avoid such obstacles many researchers have turned to model plants such as Arabidopsis thaliana (L.) Heynh., Zinnia elegans Jacq. and Nicotiana ssp., or have focused their attention on in vitro wood formation systems or in vivo approaches targeting primary meristems for transformation. Complementing such efforts, we report the use of Agrobacterium to introduce transgenes directly into cambial cells of glasshouse-grown trees in order to create transgenic somatic tissue sectors. These sectors are suitable for phenotypic evaluation and analysis of target gene function. In our experiments the wood formation zone containing the cambium of Eucalyptus, Populus and Pinus species of varying age was inoculated with Agrobacterium containing a CaMV 35S::GUS construct. Following an initial wound response, frequent and stable transformation was observed in the form of distinct GUS-staining patterns (sectors) in newly formed secondary ...Continue Reading

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Citations

Jun 23, 2018·Journal of Experimental Botany·Gerd Bossinger, Antanas V Spokevicius
Jul 4, 2007·The Plant Journal : for Cell and Molecular Biology·Antanas V SpokeviciusGerd Bossinger
Apr 29, 2015·The Plant Journal : for Cell and Molecular Biology·Fabien Baldacci-CrespMarie Baucher
Oct 20, 2018·Frontiers in Plant Science·Michael NagleSteven H Strauss
Jun 5, 2010·The New Phytologist·Rachel Spicer, Andrew Groover
Mar 1, 2011·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·V Girijashankar

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