Transformation of ginsenoside via deep eutectic solvents based on choline chloride as an enzymatic reaction medium.

Bioprocess and Biosystems Engineering
Zhu MaPei Ma

Abstract

Ginsenoside compound K (CK) with a wide range of pharmacological activities has been widely used in the healthcare product industry. However, the application of CK is limited by low productivity and difficult separation. The purpose of this study is to convert ginsenoside Rb1 into CK by improving conversion efficiency in novel "green" reaction medium-deep eutectic solvent (DES). Talaromyces purpureogenus was selected from ginseng rhizosphere soil to produce β-glucosidase with high activity and purity to transform ginsenosides, and Mn2+ was found to be an enzyme promoter. Among the DES based on choline chloride as hydrogen-bond receptor, choline chloride:ethylene glycol (ChCl:EG = 2:1) was the most promising solvent in maintaining enzyme activity and stability. In the presence of 30% v/v ChCl:EG = 2:1, the half-life of β-glucosidase was increased by 96%, the solubility of F2 was increased by 120%, and CK yield was increased by 54% compared with those in the buffer. Fourier transform infrared, circular dichroism, and fluorescence spectroscopy confirmed that DES did not destroy the structure and conformation of β-glucosidase. In addition, 80.6% CK conversion was obtained at 60 °C, pH 4.5, 48 h and 8 mM Rb1, which provided a feasib...Continue Reading

References

Dec 19, 2002·Critical Reviews in Biotechnology·Yukti BhatiaV S Bisaria
Oct 11, 2003·Biotechnology Advances·Sajja Hari Krishna
Dec 5, 2006·Biological & Pharmaceutical Bulletin·Bo-Hye KimSoon-Cheol Ahn
Apr 9, 2010·Applied Microbiology and Biotechnology·Chang-Su ParkDeok-Kun Oh
May 5, 2010·Journal of Biotechnology·Diana LindbergMikael Widersten
Sep 13, 2011·Journal of Molecular Catalysis. B, Enzymatic·Hua ZhaoShaletha Holmes
Jan 23, 2013·World Journal of Microbiology & Biotechnology·Lin-Hu QuanDeok-Chun Yang
Mar 19, 2013·Cancer Cell International·Yan ChenXiaolin Li
Jul 4, 2013·Chemosphere·Maan HayyanMohamed E S Mirghani
Oct 11, 2014·Chemical Reviews·Emma L SmithKarl S Ryder
Mar 10, 2015·Biotechnology Advances·Yu-Jin KimDeok-Chun Yang
Jun 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·András MicsonaiJózsef Kardos
Feb 13, 2016·Journal of Ginseng Research·So Jin LeeMin-Gul Kim
May 10, 2016·Journal of Ginseng Research·Yu WangWan-Taek Im
Aug 11, 2017·Bioresources and Bioprocessing·Pei XuWen-Yong Lou
Oct 13, 2017·Journal of Ginseng Research·Boyeon ParkJong-Hee Lee
Jan 10, 2018·Enzyme and Microbial Technology·Jacob EwertTimo Stressler
Aug 11, 2018·Bioresource Technology·José Carlos Santos SalgadoRichard John Ward
Oct 20, 2018·Journal of Ginseng Research·Gwi Yeong JangHeon Sang Jeong
Nov 20, 2018·Medical Journal, Armed Forces India·Mahadevan Kumar, M Mugunthan
Apr 13, 2019·Journal of Ginseng Research·Yongkun XiaoFengxie Jin
Apr 20, 2019·Trends in Biotechnology·Magdalena PätzoldDirk Holtmann
May 20, 2019·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yi ZhengHetong Lin
Sep 20, 2019·Chembiochem : a European Journal of Chemical Biology·Jun CaoErzheng Su

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Citations

Feb 20, 2021·Analytical Methods : Advancing Methods and Applications·Lin ChenJian-Hua Huang
Jul 12, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chenfei LiTiehua Zhang

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