PMID: 11607099Sep 1, 1990Paper

Transformation of Halobacterium halobium: development of vectors and investigation of gas vesicle synthesis

Proceedings of the National Academy of Sciences of the United States of America
U Blaseio, F Pfeifer

Abstract

We developed vector plasmids for the transformation of Halobacterium halobium, using the replicon region from the halobacterial phage H or from the plasmid pHH1 together with a DNA fragment conferring resistance to mevinolin. H. halobium P03, a strain lacking pHH1 as well as the restriction endonuclease activity found in wild-type H. halobium, was used as the recipient strain. All H. halobium fragments tested for autonomous replication as well as the Haloferax volcanii vector pWL102 enabled stable plasmid maintenance in this strain. A frequent loss of all vectors (including pWL102) was observed in Hf. volcanii, where >90% of the mevinolin-resistant colonies obtained after transformation had lost the vector, presumably because of restriction endonuclease activity and concomitant recombination of the mevinolin resistance marker with the chromosome. The expression of gas vesicle-encoding genes (vac) was analyzed by using a 4.5-kilobase-pair (kbp) fragment containing the plasmid-encoded p-vac gene from H. halobium or an 11-kbp fragment containing the mc-vac chromosomal gene from Haloferax mediterranei for transformation experiments with H. halobium and Hf. volcanii. These experiments indicated that the mc-vac fragment contains all ...Continue Reading

References

Sep 1, 1989·Molecular & General Genetics : MGG·M Horne, F Pfeifer
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Citations

Dec 1, 1992·Journal of Bioenergetics and Biomembranes·F Pfeifer, C Englert
Jan 5, 2005·Nature Reviews. Genetics·Thorsten Allers, Moshe Mevarech
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A S MankinR A Garrett
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C SchleperW Zillig
Feb 5, 2014·Journal of Microbiological Methods·Patrick E Gygli, Linda C DeVeaux
May 7, 2005·Archaea : an International Microbiological Journal·Steven J KaczowkaJulie A Maupin-Furlow
Sep 10, 2015·Vaccines·Shiladitya DasSarma, Priya DasSarma
Feb 5, 2015·Life·Felicitas Pfeifer
Nov 18, 2014·Life·Alison W S LukRicardo Cavicchioli
Feb 24, 2009·Journal of Microbiological Methods·Daryl P Holland, Anthony E Walsby
Jun 28, 2008·Journal of Microbiological Methods·M Hammelmann, Jörg Soppa
Nov 15, 2008·Molecular Microbiology·Torsten Hechler, Felicitas Pfeifer
Sep 20, 1992·Journal of Molecular Biology·C EnglertF Pfeifer
Nov 5, 1999·Protein Expression and Purification·G J TurnerM C Betlach
Jun 14, 2001·Systematic and Applied Microbiology·Y Luo, A Wasserfallen
Apr 16, 2013·Archaea : an International Microbiological Journal·Kate PorterMike Dyall-Smith
Jul 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Adit NaorUri Gophna
Sep 21, 2013·Annual Review of Genetics·Joel A FarkasThomas J Santangelo
Feb 9, 1993·Biochemistry·G J TurnerR M Stroud
Feb 20, 2015·Microbiology and Molecular Biology Reviews : MMBR·Haina WangQunxin She
Nov 15, 2011·Applied and Environmental Microbiology·Tao ZhengYulong Shen

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