Transformation of L-tyrosine to L-dopa by a novel fungus, Acremonium rutilum, under submerged fermentation.

Current Microbiology
R KrishnaveniY F Neelgund

Abstract

The present study deals with the transformation of L-tyrosine to L-dopa by Acremonium rutilum, a fungal tyrosinase producer, isolated from decomposed banana stud. This appears to be the first report on A. rutilum as a polyphenoloxidase producer with both cresolase and catecholase activity. Enriched Czapek-Dox agar was used for plate assay screening. Enriched potato dextrose broth was used for optimization studies, which induced high levels of L-dopa under submerged fermentation. A. rutilum gave the maximum L-dopa production (0.89 mg/ml) and tyrosinase activity (1095 U/mg) under the optimized parameters, that is, a temperature of 25 degrees C, pH 5.5, an inoculum size of 2.5 ml, and an incubation time of 72-120 h, with L-tyrosine (5 mg/ml) as substrate. Five resolved bands, with R(f) values of 0.73, 0.60, 0.54, 0.37, and 0.26, were observed, which confirmed the presence of L-dopa. This study involves the elevated profile of L-dopa production. Such study is needed, as L-dopa has the ability to control Parkinson's disease.

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Citations

Oct 22, 2010·Amino Acids·Shripad N Surwase, Jyoti P Jadhav
Apr 19, 2013·World Journal of Microbiology & Biotechnology·Panchanathan ManivasaganSe-Kwon Kim
Feb 11, 2012·Applied Biochemistry and Biotechnology·Shripad N SurwaseJyoti P Jadhav
Jun 5, 2014·Biochemistry Research International·Kamal Uddin ZaidiIshrat Naaz
Apr 23, 2011·Journal of Industrial Microbiology & Biotechnology·Ana Joyce MuñozGuillermo Gosset
Sep 14, 2012·Microbial Biotechnology·Shripad N SurwaseJyoti P Jadhav
Nov 30, 2014·Applied Microbiology and Biotechnology·Kyoungseon MinYoung Je Yoo
May 6, 2016·Neuromolecular Medicine·Liting HangKah-Leong Lim
Jul 16, 2016·Scientific Reports·Tao WeiJian-Zhong Liu
Sep 20, 2019·Journal of Industrial Microbiology & Biotechnology·Weizhu ZengJingwen Zhou
Oct 3, 2018·Journal of Chemical Technology and Biotechnology·Rita de Cássia S SousaJoão A P Coutinho
Nov 12, 2021·Applied Microbiology and Biotechnology·Eldin KurpejovićBerna Sariyar Akbulut

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