Transformation of Low Concentrations of 3-Chlorobenzoate by Pseudomonas sp. Strain B13: Kinetics and Residual Concentrations.

Applied and Environmental Microbiology
M E TrosA J Zehnder

Abstract

The transformation of 3-chlorobenzoate (3CB) and acetate at initial concentrations in the wide range of 10 nM to 16 mM was studied in batch experiments with Pseudomonas sp. strain B13. Transformation rates of 3CB at millimolar concentrations could be described by Michaelis-Menten kinetics (K(infm), 0.13 mM; V(infmax), 24 nmol (middot) mg of protein(sup-1) (middot) min(sup-1)). Experiments with nanomolar and low micromolar concentrations of 3CB indicated the possible existence of two different transformation systems for 3CB. The first transformation system operated above 1 (mu)M 3CB, with an apparent threshold concentration of 0.50 (plusmn) 0.11 (mu)M. A second transformation system operated below 1 (mu)M 3CB and showed first-order kinetics (rate constant, 0.076 liter (middot) g of protein(sup-1) (middot) min(sup-1)), with no threshold concentration in the nanomolar range. A residual substrate concentration, as has been reported for some other Pseudomonas strains, could not be detected for 3CB (detection limit, 1.0 nM) in batch incubations with Pseudomonas sp. strain B13. The addition of various concentrations of acetate as a second, easily degradable substrate neither affected the transformation kinetics of 3CB nor induced a de...Continue Reading

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Citations

Dec 4, 2019·Environmental Science and Pollution Research International·Paweł MuszyńskiTadeusz Paszko
Jun 24, 2004·Biotechnology and Bioengineering·Meltem Urgun-DemirtasBenjamin C Stark
Jan 1, 2010·Applied Microbiology and Biotechnology·Jasperien De WeertAlette Langenhoff
Jun 1, 2007·Indian Journal of Microbiology·G Banta, R S Kahlon
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Alfredo GallegoSonia E Korol
Mar 1, 1998·Microbiology·Ian M Head
Oct 9, 2001·Journal of Contaminant Hydrology·J ParkL M Abriola
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·K Kovárová-Kovar, T Egli
Aug 23, 2021·Journal of Hazardous Materials·Laura PiaiAlbert van der Wal
Nov 17, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Michael B SalernoDarrell Velegol

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