PMID: 7317353Nov 24, 1981Paper

Transformation of mammary cytoplasmic glucocorticoid receptor under cell-free conditions

Biochemistry
W A McBlainG Shyamala

Abstract

The transformation of glucocorticoid--receptor complex in the cytosol from lactating mouse mammary tissue was studied by using elevated temperature and KCl as promoters of the transformation reaction. The transformed receptor was identified from the nontransformed receptor by the following criteria: (a) increased binding to DNA--cellulose, (b) increased binding to ATP--Sepharose, (c) higher affinity for the steroid as determined by steroid dissociation kinetics, and (d) different sedimentation profiles on sucrose gradients containing KCl and sodium molybdate. A greater percentage of the nontransformed receptor was converted to the transformed state by an increased KCl concentration as opposed to increased temperature. Pretreatment of cytosol with 10 mM sodium molybdate prevented both the temperature- and salt-mediated transformation of the receptor.

References

Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·V K Moudgil, D O Toft
Jun 1, 1976·Molecular and Cellular Endocrinology·P D Colman, P Feigelson
Mar 1, 1979·Molecular and Cellular Endocrinology·R C Mierendorf, G C Mueller
Aug 1, 1979·Journal of Steroid Biochemistry·M E Mirás, R W Harrison
Feb 1, 1977·Endocrinology·J L Middlebrook, L Aronow
May 17, 1977·Biochemistry·J A GoidlG Litwack
Apr 27, 1977·Biochimica Et Biophysica Acta·J Carlstedt-DukeO Wrange
Sep 25, 1973·Journal of Molecular Biology·G G RousseauG M Tomkins
Dec 4, 1973·Biochemistry·E MilgromE E Baulieu
Sep 26, 1972·Biochemical and Biophysical Research Communications·E R DeSombreE V Jensen
Aug 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·W G JuergensJ J Elias
Oct 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·F E Stockdale, Y J Topper
Jan 1, 1968·The Journal of Clinical Endocrinology and Metabolism·S G Korenman
Sep 1, 1980·Archives of Biochemistry and Biophysics·H NishigoriD Toft
Jan 8, 1980·Biochemistry·H Nishigori, D Toft
Jul 1, 1980·Archives of Biochemistry and Biophysics·R D Marković, G Litwack

❮ Previous
Next ❯

Citations

Apr 1, 1984·Journal of Steroid Biochemistry·R E MüllerH H Wotiz
Jun 1, 1984·Journal of Steroid Biochemistry·W A McBlain, G Shyamala
Jan 1, 1984·Journal of Steroid Biochemistry·R K PuriD O Toft
Aug 1, 1985·Journal of Steroid Biochemistry·V K Moudgil, V M Caradonna
Jul 1, 1985·Journal of Steroid Biochemistry·W de BoerM Gruber
Jan 1, 1988·Journal of Steroid Biochemistry·P J WeatherillA E Wakeling
Feb 1, 1988·Journal of Steroid Biochemistry·M Pons, D Gagne
Jan 1, 1988·Journal of Steroid Biochemistry·W DietrichE Heise
Jul 1, 1982·Molecular and Cellular Endocrinology·M KaufmanA Dorato
Nov 1, 1984·Molecular and Cellular Endocrinology·G G Rousseau
Jul 1, 1987·Molecular and Cellular Endocrinology·A M LeseneyJ J Befort
Feb 28, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Sergio Li CalziEdwin R Sánchez
Sep 30, 1985·Biochemical and Biophysical Research Communications·T NemotoM Ota
Mar 30, 1984·Biochemical and Biophysical Research Communications·P A BellP J Weatherill
Feb 1, 1986·European Journal of Cancer & Clinical Oncology·W DietrichE Heise

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.