Transformation of NIH 3T3 fibroblasts by an activated form of p59hck.

Molecular and Cellular Biology
S F ZieglerR M Perlmutter

Abstract

Phosphorylation of a tyrosine residue near the carboxy terminus of src-family protein tyrosine kinases is believed to regulate the biological activity of these gene products. Conversion of this tyrosine in p59hck (Tyr-501) to a phenylalanine residue by using oligonucleotide-directed mutagenesis yielded a product (p59hckF501) with very potent transforming activity. Quantitative analysis by a soft-agar cloning assay revealed that p59hckF501 was more than 100-fold more effective than a closely related transforming element, p56lckF505, in colony formation. Cells bearing p59hckF501 had increased levels of protein phosphotyrosine. The ability of p59hckF501 to transform NIH 3T3 cells was abolished by a second mutation believed to destroy the ATP-binding domain.

References

Dec 1, 1986·Molecular and Cellular Biology·J A Cooper, C S King
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R D HuhnA R Frackelton
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J A Cooper, A MacAuley
Aug 1, 1985·Cell·J M Bishop
Jan 1, 1985·Annual Review of Biochemistry·T Hunter, J A Cooper
Nov 1, 1988·The Journal of Experimental Medicine·S F ZieglerR M Perlmutter
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T KawakamiK C Robbins
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K E Amrein, B M Sefton
Jun 1, 1987·Molecular and Cellular Biology·S F ZieglerR M Perlmutter

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Citations

Jul 21, 2010·Genes, Chromosomes & Cancer·Ruth N MackinnonLynda J Campbell
Dec 31, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Renaud PoinclouxVéronique Le Cabec
May 16, 2007·Journal of Cellular Physiology·Renaud PoinclouxVéronique Le Cabec
Oct 1, 1992·European Journal of Haematology·C OetkenT Mustelin

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