Transformation of Penicillium chrysogenum with selection for increased resistance to benomyl

FEMS Microbiology Letters
T M Picknett, G Saunders

Abstract

Incubation of protoplasts of Penicillium chrysogenum with the plasmid pBT-3 permitted selection of transformants on the basis of increased resistance to the anti-fungal agent benomyl. Transformants were obtained at a frequency of 1-2 per microgram of DNA. Southern analysis revealed that transformation had occurred by integration of vector sequences into the host genome.

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