Transformation of primary chick embryo fibroblasts by Marek's disease virus

Virology
C BuranathaiC Grose

Abstract

Marek's disease virus (MDV) is an alphaherpesvirus, which can mediate the malignant transformation of lymphocytes to form lymphomas in chickens. In this study, we demonstrate that MDV can transform primary chick embryo fibroblasts (CEF). The cell line derived from primary CEF infected with the GA strain of MDV was called CEM(MDV). The fibroblast nature of CEM(MDV) was verified by absence of cytokeratin type II. The CEM(MDV) phenotype differed from either primary CEF or MDV-infected CEF. CEM(MDV) were extensively vacuolated, with unusual multilamellar structures in the cytoplasm, The nuclei were considerably larger than those in primary CEF and were uniformly positive for proliferating cell nuclear antigen. The cell line was subcultured for more than 10 generations; however, CEM(MDV) did not support a fully productive MDV infection, because complete nucleocapsids were not detected and infectivity assays showed that cell line produced no infectious virus. PCR analyses demonstrated that this cell line carried both polypeptide 38 (pp38) and Meq DNA, MDV-specific genes associated with transformation. In addition, examination by laser scanning confocal microscopy revealed that CEM(MDV) constitutively produced MDV MEQ protein in nucle...Continue Reading

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Citations

Oct 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alon M LevyHsing-Jien Kung
Feb 9, 2010·Developmental and Comparative Immunology·Niroshan Thanthrige-DonShayan Sharif
Jun 19, 2002·The Journal of Veterinary Medical Science·Kyung-Soo ChangMisao Onuma

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