Transformation of Pseudomonas aeruginosa by electroporation

Analytical Biochemistry
J M DiverP A Sokol

Abstract

Optimum conditions were defined for the electrotransformation of Pseudomonas aeruginosa PAO1 with plasmid pLAFR1, resulting in a 1500-fold increase in transformation efficiency compared to conventional chemical transformation with MgCl2. In addition, PAO236 and two out of three recent clinical isolates of P. aeruginosa from the sputum of cystic fibrosis patients were successfully transformed with plasmid pUC19 1.8. The applied voltage and the electroporation buffer composition were shown to have the greatest effect on transformation efficiency. Freezing the cells and prolonged storage at -70 degrees C did not significantly affect the transformation efficiency. The clinical isolates tested had lower transformation efficiencies than PAO1.

Citations

Apr 5, 2002·Microbiology·Caroline WestwaterJoseph W Dolan
Sep 21, 2017·BMC Microbiology·Weiliang Huang, Angela Wilks
Mar 30, 2004·Antimicrobial Agents and Chemotherapy·Niels BaggeNiels Høiby
Apr 28, 2005·Antimicrobial Agents and Chemotherapy·Rungtip ChuanchuenHerbert P Schweizer
Jul 19, 2011·Journal of Bioenergetics and Biomembranes·Toshihede MatsunoIsao Yumoto
May 12, 2009·FEMS Microbiology Letters·Sabrina Thoma, Max Schobert
Sep 22, 2017·Microbial Cell Factories·Manuel SommerHartmut Michel
Aug 1, 1992·Journal of Bacteriology·E J HansenJ D Radolf

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.