Transformation of rhizobia with broad-host-range plasmids by using a freeze-thaw method

Applied and Environmental Microbiology
Eva Vincze, Steve Bowra

Abstract

Several species of rhizobia were successfully transformed with broad-host-range plasmids of different replicons by using a modified freeze-thaw method. A generic binary vector (pPZP211) was maintained in Mesorhizobium loti without selection and stably inherited during nodulation. The method could extend the potential of rhizobia as a vehicle for plant transformation.

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Citations

Oct 2, 2012·Indian Journal of Microbiology·Urmi Patel, Sarika Sinha
Jul 28, 2012·Molecular Plant-microbe Interactions : MPMI·Roberto MoscatielloLorella Navazio
Feb 5, 2010·Canadian Journal of Microbiology·Urmi Patel, Sarika Sinha
Dec 2, 2008·Environmental Microbiology·Geoffrey N ElliottEuan K James
Aug 27, 2010·The New Phytologist·Roberto MoscatielloLorella Navazio
Mar 12, 2016·PLoS Pathogens·Benoît Lacroix, Vitaly Citovsky
Jan 11, 2017·Journal of Bacteriology·Luyao WangVitaly Citovsky

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