PMID: 8595663Dec 1, 1995Paper

Transformation of Sordaria macrospora to hygromycin B resistance: characterization of transformants by electrophoretic karyotyping and tetrad analysis

Current Genetics
M Walz, Ulrich Kück

Abstract

The ascomycete Sordaria macrospora was transformed using different plasmid molecules containing the bacterial hygromycin B resistance gene (hph) under the control of different expression signals. The highest transformation frequency was obtained with vector pMW1. On this plasmid molecule, expression of the hph gene is directed by the upstream region of the isopenicillin N synthetase gene (pcbC) from the deuteromycete Acremonium chrysogenum. Southern analysis suggests that the vector copies are integrated as tandem repeats into the S. macrospora chromosomes and that duplicated sequences are most probably not inactivated by methylation during meiosis. Furthermore, the hygromycin B resistance (hygR) is not correlated with the number of integrated vector molecules. Electrophoretic karyotyping was used to further characterize S. macrospora transformants. Five chromosomal bands were separated by pulsed-field gel electrophoresis (PFGE) representing seven chromosomes with a total genome size of 39.5Mb. Hybridization analysis revealed ectopic integration of vector DNA into different chromosomes. In a few transformants, major rearrangements were detected. Transformants were sexually propagated to analyze the fate of the heterologous vect...Continue Reading

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Citations

Mar 18, 2006·Applied Microbiology and Biotechnology·Skander ElleucheStefanie Pöggeler
Jan 21, 2004·Fungal Genetics and Biology : FG & B·Irina N ObraztsovaEnrique Cerdá-Olmedo
Jun 26, 2002·Fungal Genetics and Biology : FG & B·Sandra MasloffUlrich Kück
Oct 19, 2010·Eukaryotic Cell·Sandra BloemendalUlrich Kück
Aug 27, 2010·European Journal of Cell Biology·Ines EnghUlrich Kück
Sep 18, 2008·Fungal Genetics and Biology : FG & B·Skander Elleuche, Stefanie Pöggeler
Mar 3, 2009·Journal of Basic Microbiology·Skander ElleucheStefanie Pöggeler
Mar 2, 2012·Molecular Microbiology·Sandra BloemendalUlrich Kück
Nov 18, 2014·AMB Express·Steffi Noack-SchönmannAnna A Gorbushina
Sep 22, 2010·Fungal Genetics and Biology : FG & B·A John Clutterbuck
Mar 3, 2012·G3 : Genes - Genomes - Genetics·Minou NowrousianUlrich Kück
Mar 13, 2020·Applied Microbiology and Biotechnology·Ines TeichertMinou Nowrousian

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