Transformation of Streptococcus pneumoniae relies on DprA- and RecA-dependent protection of incoming DNA single strands

Molecular Microbiology
Mathieu BergéJean-Pierre Claverys

Abstract

Seventy-five years after the discovery of transformation with Streptococcus pneumoniae, it is remarkable how little we know of the proteins that interact with incoming single strands in the early processing of transforming DNA. In this work, we used as donor DNA in transformation a radioactively labelled homologous fragment to examine the fate of the single-stranded (ssDNA) products of uptake in cells mutant for DprA or RecA, two proteins essential for transformation. Fifteen minutes after uptake, the labelling of specific chromosomal restriction fragments that demonstrated homologous integration in the wild type was not detected in dprA or recA cells, indicating that in the mutants incoming ssDNA could not be processed into recombinants. Investigation of the fate of donor label 1 min after uptake revealed that incoming ssDNA was immediately degraded in the absence of DprA or RecA. Our results demonstrate that incoming ssDNA requires active protection prior to the RecA-driven search for homology and that both DprA and RecA are needed for this protection.

References

Nov 1, 1978·Journal of Bacteriology·D A Morrison
Dec 11, 1992·Nucleic Acids Research·Bernard MartinJ P Claverys
Aug 1, 1988·Molecular & General Genetics : MGG·V Méjean, J P Claverys
Mar 1, 1986·Journal of Bacteriology·M N Vijayakumar, D A Morrison
Apr 11, 1973·Biochimica Et Biophysica Acta·D A Morrison, W R Guild
Sep 1, 1973·Journal of Bacteriology·D A Morrison, W R Guild
Feb 28, 1968·Journal of Molecular Biology·T Gurney, M S FOX
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D L StassiS A Lacks
Nov 1, 1983·Journal of Bacteriology·M N Vijayakumar, D A Morrison
Jan 1, 1981·Molecular & General Genetics : MGG·C W Saunders, W R Guild
Jan 1, 1995·Journal of Bacteriology·B J PearceH R Masure
Jan 1, 1994·Annual Review of Biochemistry·Stephen C Kowalczykowski, A K Eggleston
Mar 27, 1998·Molecular Microbiology·H R MasureB Spellerberg
Apr 16, 1998·Molecular Microbiology·E A CampbellH R Masure
Nov 9, 2000·Archives of Biochemistry and Biophysics·S E Steffen, F R Bryant
Sep 7, 2001·Journal of Bacteriology·J HoskinsJ I Glass
Oct 27, 2001·Applied and Environmental Microbiology·C K SungD A Morrison
Jun 18, 2002·Journal of Biomedical Science·Suh-Der TsenDarwin Han-Lin Tsen
Jul 19, 2002·Molecular Microbiology·Mathieu BergéJean-Pierre Claverys
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·R VenkateshK Muniyappa
Nov 13, 2002·The Journal of Biological Chemistry·Elizabeth A StohlH Steven Seifert
Apr 23, 2003·Trends in Microbiology·Jean-Pierre Claverys, Bernard Martin
Aug 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·M S FOX, M K ALLEN
Jul 1, 1962·Journal of Molecular Biology·S LACKS
Jan 1, 1928·The Journal of Hygiene·F Griffith

Citations

Apr 6, 2005·FEMS Microbiology Reviews·L AravindLakshminarayan M Iyer
Mar 17, 2005·Journal of Molecular Biology·Rosemary J RedfieldPaul R Langford
Jan 1, 2004·Trends in Microbiology·Tone TønjumErling Seeberg
Sep 12, 2013·Trends in Microbiology·Calum JohnstonJean-Pierre Claverys
Apr 15, 2004·Nature Reviews. Microbiology·Inês Chen, David Dubnau
Oct 28, 2006·Nucleic Acids Research·Andrew D S Cameron, Rosemary J Redfield
Jan 29, 2013·Nucleic Acids Research·Gajendradhar R DwivediDesirazu N Rao
Jun 25, 2010·Cold Spring Harbor Perspectives in Biology·Briana Burton, David Dubnau
May 29, 2008·Antimicrobial Agents and Chemotherapy·Byeonghwa JeonQijing Zhang
Mar 13, 2012·Applied and Environmental Microbiology·Kari Helene BergLeiv Sigve Håvarstein
May 18, 2010·Applied and Environmental Microbiology·A Joe ShawLee R Lynd
Jun 17, 2008·Journal of Bacteriology·Laetitia AttaiechBernard Martin
Jul 3, 2007·Journal of Bacteriology·D A MorrisonJean-Pierre Claverys
Jun 15, 2006·Annual Review of Microbiology·Jean-Pierre ClaverysBernard Martin
Jun 1, 2010·BMC Microbiology·Rasmus L Marvig, Melanie Blokesch
Nov 30, 2007·BMC Microbiology·Serkalem Tadesse, Peter L Graumann
Aug 11, 2011·PLoS Pathogens·Joshua Chang MellRosemary J Redfield
Jun 22, 2012·PLoS Pathogens·Nicholas J CroucherStephen D Bentley
Jun 3, 2014·BMC Biotechnology·Dongmei HanZhaohui Xu
Jun 4, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ankur B DaliaAndrew Camilli
Dec 3, 2005·Science·Inês ChenDavid Dubnau
Jul 3, 2007·Journal of Bacteriology·Cecilia M ArraianoJörg Vogel
Nov 29, 2015·Trends in Microbiology·Noémie Matthey, Melanie Blokesch
Aug 5, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ankur B DaliaAndrew Camilli
Nov 13, 2007·Research in Microbiology·Ola JohnsborgLeiv Sigve Håvarstein
Feb 26, 2009·Evolution; International Journal of Organic Evolution·Heather Maughan, Rosemary J Redfield
Jul 17, 2007·Molecular Microbiology·Naomi KramerDavid Dubnau
Jan 25, 2006·Molecular Microbiology·Leiv Sigve HåvarsteinJean-Pierre Claverys
Oct 30, 2013·Molecular Microbiology·Jessica M MannDavid Dubnau
Feb 3, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sandra MuschiolBirgitta Henriques-Normark
May 24, 2015·Nucleic Acids Research·Begoña CarrascoJuan C Alonso
Feb 21, 2009·FEMS Microbiology Reviews·Jean-Pierre ClaverysPatrice Polard
Feb 27, 2009·FEMS Microbiology Reviews·Jean-Francois Allemand, Berenike Maier
Sep 23, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Laetitia FontainePascal Hols
Aug 17, 2014·DNA Repair·Leonardo SorciNadia Raffaelli
May 2, 2014·Nucleic Acids Research·Johnny LisboaSophie Quevillon-Cheruel
Mar 24, 2006·FEMS Microbiology Letters·Sandra WydauEmmanuelle Maguin
Sep 19, 2006·FEMS Microbiology Letters·Leonard C SmeetsNora Goosen
Feb 11, 2014·Nature Reviews. Microbiology·Calum JohnstonJean-Pierre Claverys
Oct 11, 2012·Critical Reviews in Biochemistry and Molecular Biology·Dawit KidaneJuan C Alonso
Mar 5, 2016·PLoS Biology·Nicholas J CroucherChristophe Fraser
Aug 14, 2018·Nucleic Acids Research·Milena JaskólskaMelanie Blokesch
Nov 18, 2008·Journal of Bacteriology·Dongchang SunJean-Pierre Claverys
Jun 20, 2017·Applied and Environmental Microbiology·Blandine DavidPascal Hols
Aug 21, 2014·The Journal of Biological Chemistry·Tribhuwan YadavJuan C Alonso
Aug 29, 2019·Proceedings of the National Academy of Sciences of the United States of America·Isabelle DurieuxXavier Charpentier
Mar 20, 2019·Journal of Bacteriology·Jelle SlagerJan-Willem Veening
Apr 5, 2020·Nature Communications·Arnaud TatonSusan S Golden
Dec 25, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Yi YuChangting Liu
Jan 10, 2017·Frontiers in Microbiology·Edoardo ZaccariaJerry M Wells
Sep 4, 2020·FEMS Microbiology Reviews·Florent AilloudSebastian Suerbaum

Related Concepts

DprA protein, bacteria
Bacterial Proteins
DNA, Single-Stranded
Cell Surface Proteins
Rec A Recombinases
Recombination, Genetic
Streptococcus pneumoniae
Transformation, Bacterial
Transformation, Genetic

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.