Transformation of the cultivated mushroom, Agaricus bisporus, to hygromycin B resistance

Molecular & General Genetics : MGG
M D van de RheeH Mooibroek

Abstract

Application of biotechnology to the cultivated mushroom, Agaricus bisporus, has been hampered thus far by the lack of a transformation system. Here, transformation of both a homo- and a heterokaryotic strain of A. bisporus to hygromycin B resistance is described. Transforming DNA was integrated into the A. bisporus genome and stably maintained throughout vegetative growth. Transformants of the heterokaryotic strain formed transgenic fruiting bodies. Promoters derived from the unrelated ascomycete Aspergillus nidulans and from A. bisporus itself, were able to drive expression of the hygromycin B resistance gene. Expression controlled by a fragment of 265 bp from the A. bisporus GPD promoter was sufficient to generate transformants. However, transformation efficiency was not enhanced by using this homologous promoter.

References

Jun 1, 1990·Molecular & General Genetics : MGG·H MooibroekJ G Wessels
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R H Schiestl, T D Petes
Apr 1, 1987·Applied and Environmental Microbiology·A J CastleJ B Anderson
Jan 1, 1985·Molecular & General Genetics : MGG·E J MullaneyW E Timberlake
Apr 1, 1968·Experimental Cell Research·O L GamborgK Ojima
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M H KumagaiL K Grill
May 1, 1993·Molecular & General Genetics : MGG·H S Judelson
Jul 1, 1994·Applied and Environmental Microbiology·A LiP A Horgen

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Citations

Apr 30, 2008·Current Microbiology·Xing-gang LiaoYan Pei
Oct 19, 1999·FEMS Microbiology Reviews·C Soler-RivasH J Wichers
Jun 22, 2002·FEMS Microbiology Letters·Masahide Sunagawa, Yumi Magae
Sep 22, 2009·Applied and Environmental Microbiology·Sreedhar KilaruGary D Foster
Sep 30, 2000·Applied and Environmental Microbiology·X ChenC P Romaine
Sep 26, 2008·Science in China. Series C, Life Sciences·LiQiong GuoJunFang Lin
Dec 11, 2007·Journal of Microbiological Methods·Chun-Yi Kuo, Ching-Tsan Huang
Feb 15, 2005·Fungal Genetics and Biology : FG & B·C BurnsG D Foster
Feb 28, 2006·FEMS Microbiology Letters·Gang LiBaojian Li
Sep 22, 1998·Nature Biotechnology·N Dunn-Coleman, H Wang
Apr 22, 2015·Mycobiology·Sinil KimHyeon-Su Ro

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