Transformation of the Transcriptomic Profile of Mouse Periocular Mesenchyme During Formation of the Embryonic Cornea

Investigative Ophthalmology & Visual Science
Justin Ma, Peter Lwigale

Abstract

Defects in neural crest development are a major contributing factor in corneal dysgenesis, but little is known about the genetic landscape during corneal development. The purpose of this study was to provide a detailed transcriptome profile and evaluate changes in gene expression during mouse corneal development. RNA sequencing was used to uncover the transcriptomic profile of periocular mesenchyme (pNC) isolated at embryonic day (E) 10.5 and corneas isolated at E14.5 and E16.5. The spatiotemporal expression of several differentially expressed genes was validated by in situ hybridization. Analysis of the whole-transcriptome profile between pNC and embryonic corneas identified 3815 unique differentially expressed genes. Pathway analysis revealed an enrichment of differentially expressed genes involved in signal transduction (retinoic acid, transforming growth factor-β, and Wnt pathways) and transcriptional regulation. Our analyses, for the first time, identify a large number of differentially expressed genes during progressive stages of mouse corneal development. Our data provide a comprehensive transcriptomic profile of the developing cornea. Combined, these data serve as a valuable resource for the identification of novel regu...Continue Reading

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Citations

Sep 25, 2020·Experimental Eye Research·Aurélien TiduVincent M Borderie
Feb 9, 2021·Molecular Genetics and Metabolism Reports·Bharesh K ChauhanKen K Nischal
Dec 6, 2020·Stem Cell Research & Therapy·Nicole Ming SieJodhbir S Mehta
May 15, 2021·The Biochemical Journal·Yanel Bernardi, Pablo Hernán Strobl-Mazzulla
Aug 28, 2021·International Journal of Molecular Sciences·Haydn WalkerJudith A West-Mays
Oct 12, 2020·Experimental Eye Research·Haydn WalkerJudith A West-Mays
Aug 10, 2021·Disease Models & Mechanisms·Chloe M StantonVeronique Vitart

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Datasets Mentioned

BETA
GSE121044

Methods Mentioned

BETA
PCR
RNA-Seq
in vitro transcription

Software Mentioned

Bowtie
NOISeq

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