PMID: 8593690Nov 1, 1995Paper

Transformations of Penicillium islandicum and Penicillium frequentans that produce anthraquinone-related compounds

Current Genetics
K X HuangU Sankawa

Abstract

Wild-type strains of Penicillium islandicum and Penicillium frequentans, which produce anthraquinone and related compounds, were transformed to benomyl and hygromycin B resistance. Plasmids pSV50 and pBT6, with benomyl-resistant beta-tublin genes, and plasmids pAN7-1 and pDH25, with a bacterial hygromycin phosphotransferase gene under the control of Aspergillus nidulans sequences, were used respectively. Transformation frequencies with these plasmids were 10-20 transformants per micrograms of DNA per 4-8 x 10(7) viable protoplasts. Integration of plasmid DNAs into chromosomal DNAs was confirmed by Southern-blot analysis. Copy numbers and sites of integration varied among transformants. The integrated plasmid DNAs conferring a drug-resistant phenotype were mitotically stable with or without selection. The demonstration of such transformation systems in the essential first step in the application of recombinant DNA technology to study the biosynthetic genes of anthraquinone and related compounds in P. islandicum and P. frequentans.

References

Oct 1, 1992·The Journal of Antibiotics·H OhashiJ Mochizuki
Jan 1, 1990·Current Genetics·S WnendtU Stahl
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J WangS A Leong
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M M YeltonW E Timberlake
Dec 1, 1983·Gene·J TilburnR W Davies
Jan 1, 1953·Advances in Genetics·G PONTECORVOA W J BUFTON
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S J Vollmer, C Yanofsky

❮ Previous
Next ❯

Citations

Jul 5, 2016·Chemical & Pharmaceutical Bulletin·Nobuyuki KashiwaIsao Fujii

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.