Transforming ability of Gag-Myc fusion proteins correlates with Gag-Myc protein stability and transcriptional repression

Oncogene
W Law, M L Linial

Abstract

Avian retroviruses that have transduced c-myc are useful tools to study the conditions necessary for cellular transformation. FH3, one such retrovirus which encodes a Gag-Myc fusion protein, is not transforming in quail embryonic fibroblasts, but a late variant of FH3 that arose after passaging FH3-infected cells is transforming. Mutational analysis of FH3 revealed that the presence of a portion of the retroviral protease in FH3 inhibited transformation and that this inhibition was transferable to a more highly transforming retrovirus, MC29. Transforming and non-transforming FH3-derived and MC29-derived Gag-Myc proteins were used to further explore characteristics of Myc necessary for transformation. Gag-Myc proteins which were transforming were found to be the most stable in the cell. To distinguish whether transactivation and/or repression is correlated to transformation, the various Gag-Myc fusion proteins were tested for their ability to activate or repress c-Myc targets. Results indicated that a correlation exists between transforming Gag-Myc proteins and their ability to repress, whereas all Gag-Myc proteins could transactivate, regardless of their ability to transform. Taken together, these results suggest that protein s...Continue Reading

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Citations

May 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Markus WelckerBruce E Clurman
Oct 20, 2009·BMC Bioinformatics·Andrija TomovicEdward J Oakeley
Jul 19, 2012·PloS One·Gustavo A BarisoneElva Díaz
Sep 6, 2002·Oncogene·M Christine Hollander, Albert J Fornace

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