Transforming growth factor alpha and a PC12-derived growth factor induce neurites in PC12 cells and enhance the survival of embryonic brain neurons

Cell Regulation
M ZhangB D Howard

Abstract

We have identified and characterized a 5000-Da protein that induces neurite outgrowth from PC12 pheochromocytoma cells, enhances the survival of embryonic rat brain neurons in primary culture, and induces the multiplication of embryonic rat brain astrocytes in primary culture. The factor is produced by a flat cell PC12 variant that expresses the activated ras oncogene after transfection of the gene. The factor resembles transforming growth factor alpha (TGF alpha) and epidermal growth factor (EGF) in that it induces anchorage-independent colony formation of normal rat kidney cells in soft agar and competes with EGF for binding to the EGF receptor. Rat TGF alpha and human TGF alpha also induce neurite outgrowth from PC12 and enhance the survival of embryonic brain neurons. The PC12 variant-derived factor can be distinguished from TGF alpha and EGF immunologically and by migration rates on reversed-phase high-performance liquid chromatography.

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Jan 1, 1987·Annual Review of Cell Biology·T F Deuel
Jan 1, 1986·Annual Review of Biochemistry·D H Hamer
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M D Hollenberg, P Cuatrecasas
May 1, 1981·The Journal of Cell Biology·P W GunningE M Shooter

❮ Previous
Next ❯

Citations

Jun 8, 2000·International Journal of Cancer. Journal International Du Cancer·K TaniguchiT Mayumi
Jun 28, 2000·Progress in Neurobiology·M P Junier
Nov 13, 2002·Annual Review of Physiology·Mary C Williams
Apr 24, 1997·Annals of the New York Academy of Sciences·F PoussetP E Keane
Mar 28, 2013·Applied Immunohistochemistry & Molecular Morphology : AIMM·Nelson G Ordóñez
Jun 16, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiajun ZhangWayne Y W Lee
Jun 13, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jörg WiltingJochen Rössler
Jul 27, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M I RamirezM C Williams
Dec 1, 1993·Journal of Neuroscience Research·C F WuB D Howard
Mar 27, 2018·Cancer Science·Harini KrishnanGary S Goldberg
Jan 1, 1991·Neuroscience and Biobehavioral Reviews·C R Plata-Salamán

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.