PMID: 9432049Jan 31, 1998Paper

Transforming growth factor-beta 1 delays formation of granulocyte-macrophage colony-forming cells, but spares more primitive progenitors during ex vivo expansion of CD34+ haemopoietic progenitor cells

British Journal of Haematology
A GarbeR Henschler

Abstract

Ex vivo culture and expansion of autologous haemopoietic transplants has been developed to improve tumour cell purging and accelerate haemopoietic reconstitution by transplantation of increased progenitor cell numbers. We studied the effect of the negative haemopoietic regulator, transforming growth factor beta-1 (TGF-beta 1) on primitive precursors during ex vivo expansion of CD34+ cells. When added directly to methylcellulose colony-forming assays, TGF-beta 1 potently suppressed the development of granulocyte-macrophage colonies from CD34+ enriched peripheral blood progenitor cells (80-90% inhibition). In contrast, expansion of total nucleated cells and granulocyte-macrophage colony-forming cells (GM-CFC) from CD34+ progenitors in liquid culture in the presence of stem cell factor (SCF), interleukin (IL)-1 beta, IL-3, IL-6 and erythropoietin (EPO) was inhibited to 32-65% of control culture levels within 14 d when TGF-beta 1 was added, and still produced an average 3.3-fold absolute amplification of GM-CFC. The inhibitory effect of TGF-beta 1 on GM-CFC generation was reversed when it was washed out on day 6 of ex vivo expansion cultures, and total numbers of GM-CFC generated from expansion cultures then reached levels of untre...Continue Reading

Citations

Apr 6, 1999·The Journal of Experimental Medicine·M BhatiaJ E Dick
Oct 5, 2007·Blood·Ulrika BlankStefan Karlsson
Aug 27, 2005·Oncogene·Jonas Larsson, Stefan Karlsson
Feb 4, 2011·Experimental Hematology·June Li
Dec 25, 2010·Birth Defects Research. Part C, Embryo Today : Reviews·Suleyman Coskun, Karen K Hirschi
Oct 5, 2010·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Jun Seita, Irving L Weissman
Apr 3, 2015·Blood·Ulrika Blank, Stefan Karlsson
May 15, 2015·Systems and Synthetic Biology·Anuradha Vaidya, Vaijayanti P Kale
Aug 18, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Snjezana MartinovicSlobodan Vukicevic
Jan 19, 2000·Leukemia·A SolanillaJ Ripoche
Jan 26, 2021·Journal of Cellular Physiology·Dushyant Kumar GautamIndira Paddibhatla

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