Transforming growth factor-beta 1 induces apoptosis in gastric cancer cells through a p53-independent pathway

Cancer
M YamamotoK Sugimachi

Abstract

Apoptosis is induced by various anticancer agents or radiation through the tumor suppressor gene p53-dependent pathway and is also induced by other factors, including transforming growth factor-beta 1 (TGF-beta 1). In this study, the authors investigated whether TGF-beta 1 would induce apoptosis in gastrointestinal cancer cells, and its relation to the status of the p53 gene. The induction of apoptosis by TGF-beta 1 was determined in 12 gastrointestinal cancer cell lines using DNA ladder formation. Status of the p53 gene was examined by sequencing of cDNA from p53 mRNA and expressions of TGF-beta 1 mRNA and TGF receptors I and II mRNAs were determined by Northern blot analysis and the reverse transcriptase-polymerase chain reaction analysis, respectively. Of 12 cell lines, wild-type p53 was present in 3 lines, point mutation was detected in 7 lines, and p53 mRNA was absent in 2 lines. TGF-beta 1 was expressed in all 12 lines, but both TGF receptors I and II were expressed in only 6 lines. Addition of TGF-beta 1 induced DNA ladder formation only in KATOIII cells, with deleted p53 mRNA, but expressed TGF receptors I and II. These findings show that TGF-beta 1 induces apoptosis in gastric cancer cells through TGF-beta receptors I ...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S J KuerbitzM B Kastan
Jan 1, 1992·Annual Review of Immunology·J J CohenK S Sellins
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F A OberhammerR Schulte-Hermann
Jan 1, 1991·International Review of Experimental Pathology·M J Arends, A H Wyllie
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K E NoonanI B Roninson
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R J RotelloL E Gerschenson
Jan 1, 1990·Annual Review of Cell Biology·J Massagué
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D R WelchM Nakajima
Nov 1, 1987·The Journal of Experimental Medicine·G LeeP W Kincade
Feb 1, 1989·Japanese Journal of Cancer Research : Gann·M MukaiH Akedo
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A B RobertsM B Sporn
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A B RobertsM B Sporn
Oct 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K FukudaJ F Chiu
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P Jansen-DürrM Eilers
Mar 1, 1993·Immunology Today·J J Cohen

❮ Previous
Next ❯

Citations

Mar 16, 2013·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Ayako NakamuraKazuhiko Hayashi
Jul 1, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B SaileG Ramadori
Nov 21, 2001·Japanese Journal of Cancer Research : Gann·M MasutaniY Kawakami
Jun 20, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Eunice Ribeiro de Andrade SáPatrícia Gama
Feb 11, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Satoru NodaKosei Hirakawa
Sep 22, 2005·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·M J van ErkB van Ommen
Dec 8, 2007·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·K TriantafyllouS D Ladas
Dec 11, 2007·Regulatory Peptides·Eunice Ribeiro de Andrade SáPatrícia Gama
Nov 20, 2015·Frontiers in Cellular and Infection Microbiology·Nianshuang LiNong-Hua Lu
Mar 15, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Isabella ApriglianoBernhard Saile
Nov 25, 1997·Japanese Journal of Cancer Research : Gann·O TominagaT Muto
Mar 4, 2000·Microscopy Research and Technique·A von Herbay, J Rudi
Jan 8, 1998·International Journal of Cancer. Journal International Du Cancer·C VindigniP Tosi
Feb 7, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jürgen KnoblochUlrich Rüther
Sep 1, 2005·Clinical Orthopaedics and Related Research·R Lor RandallDavid E Joyner
Jan 26, 2005·Acta Neuropathologica·Wolf MuellerAndreas von Deimling
Jun 1, 1997·Molecular and Cellular Biology·B LiA S Perkins

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis