PMID: 9178100Jun 15, 1997Paper

Transforming growth factor-beta 1 regulation of bone sialoprotein gene transcription: identification of a TGF-beta activation element in the rat BSP gene promoter

Journal of Cellular Biochemistry
Y OgataJ Sodek

Abstract

Transforming growth factor-beta (TGF-beta) increases steady-state mRNA levels of several extracellular matrix proteins in mineralized connective tissues. Bone sialoprotein (BSP) is a major constituent of the bone matrix, thought to initiate and regulate the formation of mineral crystals. To determine the molecular pathways of TGF-beta 1 regulation of bone proteins, we have analyzed the effects of the TGF-beta 1 on the expression of the BSP in the rat osteosarcoma cell line (ROS 17/2.8). TGF-beta 1 at 1 ng/ml, increased BSP mRNA levels in ROS 17/2.8 cells approximately 8-fold: the stimulation was first evident at 3 hr, reached maximal levels at 12 hr and slowly declined thereafter. Since the stability of the BSP mRNA was not significantly affected by TGF-beta 1, and nuclear "run-on" transcription analyses revealed only a approximately 2-fold increase in the transcription of the BSP gene, most of the increase in BSP mRNA appeared to involve a nuclear post-transcriptional mechanism. Moreover, the effects of TGF-beta 1 were indirect, since the increase in BSP mRNA was abrogated by cycloheximide (28 micrograms/ml). To identify the site of transcriptional regulation by TGF-beta 1, transient transfection analyses were performed using ...Continue Reading

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Citations

Mar 17, 2004·Biochemical and Biophysical Research Communications·Qisheng TuJake Jinkun Chen
Dec 4, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·W SunB D Smith
Jul 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Miki HimenoToshihisa Komori
Apr 8, 2004·Journal of Periodontology·Emi ShimizuYorimasa Ogata
Jul 1, 2010·Journal of Oral Science·Xinyue LiYorimasa Ogata
Apr 7, 2011·Journal of Oral Science·Yoko SasakiYorimasa Ogata
Dec 20, 2002·Connective Tissue Research·Marion LucchiniJean-Christophe Farges
Dec 26, 2012·Gene·Liming ZhouYorimasa Ogata
Feb 28, 2008·Journal of Periodontal Research·Y Ogata
Apr 18, 2014·Journal of Oral Science·Hiroyoshi Matsumura, Yorimasa Ogata
Mar 15, 2013·Journal of Oral Science·Liming Zhou, Yorimasa Ogata
Sep 18, 2013·Journal of Oral Science·Hideki TakaiYorimasa Ogata
Mar 19, 2014·Journal of Bone and Mineral Metabolism·Hiroyoshi MatsumuraYorimasa Ogata
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B GanssJ Sodek
Sep 15, 2006·Biochemical and Biophysical Research Communications·Anders P RehnMikael Wendel
Feb 27, 2014·Journal of Dental Research·W HeP R Cooper
Jan 24, 2007·Journal of Cellular Biochemistry·Dong-Soon KimYorimasa Ogata
Mar 28, 2007·Journal of Cellular Biochemistry·Colm Morrissey, Robert L Vessella
Dec 5, 2008·Journal of Periodontal Research·Y NakayamaY Ogata
Jun 24, 2009·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Melissa Thys, Guy Van Camp
Jun 22, 2010·Journal of Cellular Biochemistry·Xinyue LiYorimasa Ogata
Oct 15, 2005·In Vitro Cellular & Developmental Biology. Animal·Jun LiuBrian H Clarkson
Sep 28, 2005·Journal of Cellular Biochemistry·Naoko KatoYorimasa Ogata
Nov 19, 2005·Journal of Cellular Biochemistry·Yu NakajimaYorimasa Ogata
Apr 12, 2012·Journal of Cellular Biochemistry·Xinyue LiYorimasa Ogata

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