Abstract
Multiple sclerosis (MS) is associated with high levels of circulating T lymphocytes that respond to the myelin antigens myelin basic protein (MBP) and proteolipid protein (PLP) by producing various cytokines including interferon-gamma (IFN-gamma) that makes MS worse and transforming growth factor-beta (TGF-beta), an endogenously produced immunosuppressant that might act beneficially. To further define the role of TGF-beta in MS, we examined the effects of recombinant TGF-beta 1 (rTGF-beta 1) on autoantigen-mediated regulation of cytokines in MS and myasthenia gravis (MG). Blood mononuclear cells (MNC) were cultivated with or without rTGF-beta 1, and with or without autoantigen or the recall antigen PPD. MNC expressing cytokine mRNA were detected after in situ hybridization with radiolabeled cDNA oligonucleotide probes. Femtogram concentrations of rTGF-beta 1 suppressed MBP-, PLP- and PPD-induced upregulation of IFN-gamma, IL-4, IL-6, tumor necrosis factor-alpha (TNF-alpha), TNF-beta and perforin in MS, and acetylcholine receptor (AChR)-induced augmentation of these pro-inflammatory cytokines in MG, but had no effects on autoantigen- or PPD-induced expression of IL-10 or TGF-beta itself. rTGF-beta 1 also suppressed numbers of my...Continue Reading
Citations
May 1, 1997·European Journal of Immunology·T MarthB L Kelsall
May 1, 1997·Muscle & Nerve·G X ZhangH Link
Nov 1, 1996·European Journal of Immunology·G X ZhangH Link
Jul 4, 1998·Scandinavian Journal of Immunology·D MatuseviciusH Link
May 21, 1999·Multiple Sclerosis : Clinical and Laboratory Research·G V McDonnellC A Graham
Jun 11, 2017·Journal of Neuroinflammation·K M DanikowskiB S Prabhakar
Mar 1, 1996·Clinical and Experimental Immunology·B G XiaoH Link
Feb 13, 1996·Annals of the New York Academy of Sciences·C G MaH Link
Sep 2, 1998·Acta Neurologica Scandinavica·W Z TianH Link
Nov 1, 1995·Multiple Sclerosis : Clinical and Laboratory Research·M SöderströmH Link
Jan 3, 2001·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·P BongioanniG Meucci
Sep 6, 2003·Acta Neurologica Scandinavica·D ReskeH-F Petereit
Nov 19, 2011·Multiple Sclerosis International·Sara Ireland, Nancy Monson
Sep 4, 2012·International Journal of Molecular Sciences·Arpád DobolyiGábor Lovas
Mar 18, 2017·Journal of Neuroinflammation·Farideh TalebiFarshid Noorbakhsh
Jul 27, 2001·Current Opinion in Pulmonary Medicine·Y C Lee, K B Lane
Feb 24, 1998·Stroke; a Journal of Cerebral Circulation·N KostulasH Link
May 16, 2000·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·W X HuangJ Hillert
Aug 13, 2003·Autoimmunity·Robert D HollifieldMohammed K Sharief
Jul 1, 1997·Clinical Immunology and Immunopathology·J ZhuH Link
Dec 24, 1997·Clinical Immunology and Immunopathology·A DiabM Bakhiet
Jun 10, 1998·Cytokine & Growth Factor Reviews·B M Pratt, J M McPherson