PMID: 2508623Aug 1, 1989Paper

Transforming growth factor beta 1 treatment of AKR-2B cells is coupled through a pertussis-toxin-sensitive G-protein(s)

The Biochemical Journal
P H Howe, E B Leof

Abstract

Transforming growth factor beta (TGF beta 1) is a potent regulator of DNA synthesis and cellular proliferation. In this study, we investigated whether the growth stimulatory signal of TGF beta 1 is transduced intracellularly by guanine nucleotide regulatory proteins (G-proteins). In plasma membranes from AKR-2B cells, TGF beta 1 increased binding of the radiolabelled, non-hydrolysable GTP analogue, guanosine 5'-[gamma-[35S]thio]triphosphate (GTP[35S]), in a dose-dependent manner. Maximal effects occurred between 0.4 and 1.0 nM-TGF beta 1. Specific binding of GTP[35S] occurred with a Kd of 3.2 x 10(-8) M which was not affected by addition of TGF beta 1. Instead, TGF beta 1 increased the number of available binding sites for GTP[35S] from 16.2 +/- 1.2 to 21.6 +/- 2.1 pmol/mg of protein. GTP[35S] binding was both nucleotide- and growth-factor-specific. Only guanine nucleotides were able to compete for binding, and of the growth factors tested (epidermal growth factor, platelet-derived growth factor, insulin, TGF beta 1 and TGF beta 2) only TGF beta 1 affected GTP[35S] binding. TGF beta 1 increased GTPase activity, as determined by the release of 32PO4(3-) from GTP gamma[32P], from 116 +/- 5.5 to 175 +/- 4.3 pmol/mg of protein foll...Continue Reading

Citations

May 13, 1999·Journal of Neuroendocrinology·R CoyaC Diéguez
Feb 14, 1990·European Journal of Biochemistry·R M Lyons, H L Moses
Aug 19, 1991·International Journal of Cancer. Journal International Du Cancer·M T JenningsH L Moses
Jan 23, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A S BoyerR B Runyan
Feb 12, 2019·American Journal of Respiratory Cell and Molecular Biology·Christie A OjiakuReynold A Panettieri
Jan 1, 1993·Growth Factors·H Lahm, N Odartchenko
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·L C Eriksson, G N Andersson
Feb 18, 1999·Molecular and Cellular Endocrinology·J A Visser, A P Themmen
Jul 26, 1991·Journal of Immunological Methods·A Meager
Jan 22, 2003·The Journal of Investigative Dermatology·Krystyna E Rys-SikoraRaymond L Konger

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