PMID: 8592079Jan 1, 1996Paper

Transforming growth factor-beta controls cell-matrix interaction of microvascular dermal endothelial cells by downregulation of integrin expression

The Journal of Investigative Dermatology
R FrankM Heckmann

Abstract

Transforming growth factor-beta (TGF-beta) is a pleiotropic regulatory factor of tissue remodeling. Angiogenesis, a prerequisite of tissue repair and tissue expansion, is induced by TGF-beta in vivo, while proliferation and migration of cultured endothelial cells are inhibited by TGF-beta. Indirect mechanisms stimulating angiogenesis and modification of TGF-beta effects by cell-matrix interaction have been postulated to account for this paradigm. Because cellular behavior in tissue remodeling is decisively determined by cell-matrix interactions, which in turn is mediated via integrins, we investigated the effect of TGF-beta on matrix-dependent endothelial cell functions. Integrin expression of human dermal microvascular endothelial cells (HDMEC) was measured by Northern blot and fluorescence-activated cell sorter analysis after TGF-beta treatment and correlated to cell-matrix interactions, which were studied in a colorimetric cell attachment assay as well as the Boyden chamber chemotaxis assay. We found a cell-specific downregulation of integrin expression in HDMEC on the level of mRNA as well as on the cell surface. This effect correlated well with the reduction of integrin-dependent cell adhesion to several matrix proteins, i...Continue Reading

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Citations

Mar 23, 2004·Journal of Cellular Biochemistry·Aleksandar FranckiE Helene Sage
Sep 21, 2000·The Tohoku Journal of Experimental Medicine·R S Yang, T F Huang
Dec 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P J Polverini
Nov 1, 1997·Trends in Cardiovascular Medicine·P Carmeliet, D Collen
May 10, 2000·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·O E TeebkenA Haverich
Apr 25, 2006·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·S Schultze-MosgauM Thorwarth
May 1, 1997·Kidney International·P Pintavorn, B J Ballermann
Apr 7, 2009·Trends in Cardiovascular Medicine·Guido KrenningMartin C Harmsen

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