PMID: 8943771Oct 1, 1996Paper

Transforming growth factor beta in bovine milk: concentration, stability and molecular mass forms

The Journal of Endocrinology
M L RogersD A Belford

Abstract

Transforming growth factor beta (TGF-beta) is one of the predominant growth factors present in milk. The concentration, molecular mass forms and stability of TGF-beta in bovine milk were investigated using a standard bioassay measuring the growth inhibition of a milk lung epithelial cell line. Most of the TGF-beta bioactivity in milk was found to be in a latent form, which was also retained in the whey fraction. After acid activation, the total TGF-beta concentration was 4.3 +/- 0.8 ng and 3.7 +/- 0.7 ng TGF-beta per ml of milk and cheese whey respectively. Cation-exchange chromatography at pH 6.5 was used to concentrate latent whey-derived TGF-beta, which could be activated by transient exposure to extremes of pH, urea or heat. Heparin did not significantly activate milk-derived TGF-beta. Neutral gel filtration of the cationic whey fraction revealed a major peak of latent TGF-beta with a molecular mass of 80 kDa and a smaller peak at 600 kDa. Transient acidification of the cationic whey fraction prior to neutral gel filtration, or gel filtration under acidic conditions, released low molecular mass TGF-beta from both high molecular mass peaks. Whey-derived TGF-beta was purified using a five-step chromatographic procedure. An N-...Continue Reading

Citations

Jan 1, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Michael A Conlon, Frank M Tomas
May 3, 2003·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·O KolekT Motyl
Aug 22, 2001·In Vitro Cellular & Developmental Biology. Animal·V L TaylorL C Read
Sep 28, 1999·Current Problems in Pediatrics·D S Wheeler
Jan 14, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A Nakao
Feb 26, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Anthony R DyerLeanna C Read
Jun 29, 2005·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·H Miles PrinceDavid Belford
Apr 2, 2017·The Journal of Allergy and Clinical Immunology. in Practice·J Pablo AboniaAntonella Cianferoni
Sep 12, 2013·International Archives of Allergy and Immunology·Gerco den HartogR J Joost van Neerven
Apr 6, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kopperuncholan NamachivayamAkhil Maheshwari
Jan 12, 2008·Journal of the American College of Nutrition·Geoffrey W Krissansen
Jun 10, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·T E RaynerD A Belford

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