Transforming growth factor-beta-induced alpha-smooth muscle cell actin expression in renal proximal tubular cells is regulated by p38beta mitogen-activated protein kinase, extracellular signal-regulated protein kinase1,2 and the Smad signalling during epithelial-myofibroblast transdifferentiation

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Attila SebeIstvan Mucsi

Abstract

Transforming growth factor-beta (TGFbeta)-induced epithelial-myofibroblast transdifferentiation is a central mechanism contributing to the pathogenesis of progressive tubulo-interstitial fibrosis. We wanted to dissect the role of extracellular signal-regulated protein kinase (ERK1,2), p38 mitogen-activated protein kinase (p38 MAPK) and the receptor-regulated Smad proteins in the regulation of alpha-smooth muscle cell actin (alphaSMA) expression, a hallmark of myofibroblast formation, induced by TGFbeta in renal proximal tubular cells. Activation of signalling molecules was assessed by western blotting using phospho-specific antibodies. To specifically interfere with signalling cascades, porcine proximal tubular cells (LLC-PK/AT1) were infected with recombinant replication-deficient adenoviruses. In other experiments, specific kinase inhibitors were used. The alphaSMA synthesis was assessed by western blotting or immunofluorescent staining of cellular alphaSMA. To assess the regulation of the alphaSMA promoter, tubular cells were transiently transfected with a 785 bp alphaSMA promoter-luciferase reporter construct and vectors interfering with the Smad pathway. Blocking ERK1,2 activation with PD98059 or p38 MAPK with SB 203580 po...Continue Reading

Citations

Aug 6, 2010·The Journal of Biological Chemistry·Naxin GuoJames L Funderburgh
May 15, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Bin ZhuXiao-xia Cheng
Oct 21, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Rui ZengGang Xu
Dec 23, 2008·American Journal of Respiratory Cell and Molecular Biology·Liang ZhengDanielle Morse
Apr 17, 2012·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Ya-fang SongLie-ming Xu
Aug 24, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Zhigang Lv, Lieming Xu
Dec 4, 2009·Kidney International·Pranali PatelPeter J Margetts
May 22, 2016·Cellular Signalling·José M Muñoz-FélixCarlos Martínez-Salgado
Dec 9, 2017·Frontiers in Pharmacology·Xiaoyi LiRuixin Zhu
Feb 8, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Ratchadaporn NamsenSaranyapin Potikanond
Jan 10, 2013·British Journal of Pharmacology·Hoeke A BaarsmaReinoud Gosens
May 4, 2013·Cell Biology International·Jiali WeiYouming Peng
Jul 7, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ákos Gasparics, Attila Sebe
Nov 7, 2019·Expert Review of Gastroenterology & Hepatology·Rosanna Santoro, Alessandra Mangia
Aug 16, 2018·Scientific Reports·Meera SaxenaGerhard Christofori
May 23, 2014·Experimental Dermatology·Donal O'KaneCecilia M O'Kane
May 12, 2018·International Journal of Molecular Sciences·Bhavani S KowtharapuOliver Stachs
Mar 20, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Ákos GasparicsAttila Sebe
May 7, 2019·Frontiers in Pharmacology·Attila FinthaAttila Sebe
Nov 7, 2015·Cytoskeleton·Sandeep M NalluriEsther W Gomez
Jul 28, 2016·The Journal of Pharmacy and Pharmacology·Ruiyan PanMing Jin
Apr 26, 2017·Molecular Medicine Reports·Luoyuan CaoFeng Lin
May 17, 2013·International Heart Journal·Mayuko IshikawaToshihiko Ishimitsu
Nov 28, 2012·The American Journal of Pathology·Attila FinthaAttila Sebe
Jan 20, 2021·ACS Biomaterials Science & Engineering·Mohd Aslam SaifiChandraiah Godugu
May 10, 2019·Molecular Pharmaceutics·Khan AsifullahMinjie Sun

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.