Transforming growth factor-beta induces cellular injury in experimental diabetic neuropathy.

Experimental Neurology
Muragundla AnjaneyuluJames W Russell

Abstract

The mechanism/s leading to diabetic neuropathy are complex. Transforming growth factor-beta1 (TGF-beta1) has been associated with diabetic nephropathy and retinopathy but not neuropathy. In this study, changes in TGF-beta isoforms were examined in vivo and in vitro. Two groups of animals, streptozotocin diabetic with neuropathy and non-diabetic controls were examined at 4 weeks (n=10/group) and 12 weeks (n=8/group). In diabetic DRG using quantitative real-time PCR (QRT-PCR), TGF-beta1 and TGF-beta2 mRNA, but not TGF-beta3, was increased at 4 and 12 weeks. In sciatic nerve TGF-beta3 mRNA was primarily increased. Immunohistochemistry (DRG) and immunoblotting (sciatic nerve) showed similar differential protein expression. In sciatic nerve TGF-beta formed homo- and hetero-dimers, of which beta(2)/beta(3), beta(1)/beta(1), and beta(1)/beta(3) were significantly increased, while that of the TGF-beta(2)/beta(2) homodimer was decreased, in diabetic compared to non-diabetic rats. In vitro, pretreatment of embryonic DRG with TGF-beta neutralizing antibody prevents the increase in total TGF-beta protein observed with high glucose using immunoblotting. In high glucose conditions, combination with TGF-beta2>beta1 increases the percent of cl...Continue Reading

References

Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N SandersonS S Thorgeirsson
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·G VenkataramanR Sasisekharan
Aug 1, 1994·Annals of Neurology·J W RussellJ L Podratz
Mar 29, 1996·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P Anand
Jul 25, 1998·Genes & Development·Y G ChenJ Massagué
Aug 26, 1998·Biochimica Et Biophysica Acta·M Montal
Nov 10, 2001·Journal of the Neurological Sciences·J W RussellA J Windebank
Jan 26, 2002·Cell and Tissue Research·Norbert Schuster, Kerstin Krieglstein
Mar 29, 2002·Journal of Cellular Physiology·Peter Ten DijkeSusumu Itoh
May 16, 2002·The Journal of Biological Chemistry·Ahmed A SamatarChandra C Kumar
Sep 10, 2002·American Journal of Physiology. Renal Physiology·Elizabeth Gore-HyerMaria Trojanowska
Nov 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·James W RussellEva L Feldman
Apr 5, 2003·Drug News & Perspectives·Maria D. Rutkowski, Joyce A. DeLeo
Nov 5, 2003·British Journal of Pharmacology·Garth T WhitesideKatharine Walker
Jan 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Roberto BianchiPietro Ghezzi
Mar 9, 2004·The Journal of Biological Chemistry·Seiji OhashiToshio Doi
Mar 20, 2004·Journal of Neurochemistry·Alison Berent-SpillsonJames W Russell
Dec 14, 2004·International Journal of Radiation Oncology, Biology, Physics·Liguang ChenZeljko Vujaskovic
Sep 30, 2005·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·G LauriaUNKNOWN European Federation of Neurological Societies
Mar 1, 2006·Diabetes/metabolism Research and Reviews·Rodica Pop-BusuiMartin Stevens
Aug 26, 2006·Nature Clinical Practice. Neurology·James W Russell
Dec 21, 2007·Nature Reviews. Neuroscience·David R Tomlinson, Natalie J Gardiner

❮ Previous
Next ❯

Citations

Aug 19, 2008·Reviews in Endocrine & Metabolic Disorders·Claudia Figueroa-RomeroEva L Feldman
Mar 12, 2009·The Journal of Clinical Endocrinology and Metabolism·John DoupisAristidis Veves
Mar 30, 2011·Drugs·Howard S Smith, Charles E Argoff
Oct 3, 2009·Journal of Diabetes and Its Complications·Juan YbarraJeroni Jurado
Sep 26, 2013·Clinical Science·Aaron D McClelland, Phillip Kantharidis
Jan 11, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Shoko Nakayama-IchiyamaToshiaki Hanafusa
May 30, 2020·International Journal of Molecular Sciences·Krish ChandrasekaranJames William Russell
May 7, 2015·American Journal of Physiology. Endocrinology and Metabolism·Krish ChandrasekaranJames W Russell
Jan 4, 2018·Annals of Clinical and Translational Neurology·Krish ChandrasekaranJames W Russell
Apr 27, 2010·The Journal of Pain : Official Journal of the American Pain Society·Kanwaljit ChopraAnurag Kuhad

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis