Transforming growth factor-beta (TGF-beta) stimulates the expression of beta1 integrins and adhesion by rat mesangial cells

Experimental Cell Research
S KagamiY Kuroda

Abstract

Transforming growth factor-beta (TGF-beta) has been implicated in the pathogenesis of mesangial matrix (MM) accumulation in human and experimental glomerulonephritis. To clarify molecular mechanisms responsible for pathological MM deposition, we examined the effect of TGF-beta on the production of beta1 integrins and on adhesion function of rat mesangial cells (MC). In immunoprecipitation experiments using [35S]methionine-labeled MC, stimulation of MC with TGF-beta for 48 h resulted in an increase in the synthesis of alpha1beta1 (collagen/laminin receptor) and alpha5beta1 (fibronectin receptor) integrins accompanied by increases in the synthesis of their ligands, collagen type I (collagen I), and fibronectin. A time-dependent increase in beta1, alpha1 integrin subunit mRNA peaking 48 h after exposure to TGF-beta was shown by Northern blot analysis. After 48 h of treatment with TGF-beta, MC displayed significant increases in adhesion to fibronectin, collagen I, and laminin as compared to untreated MC. Anti-beta1 antiserum significantly inhibits MC adhesion to fibronectin, collagen I, and laminin. Anti-alpha1 subunit antibody very strongly inhibited adhesion to collagen I and laminin, but not to fibronectin. Synthetic peptides co...Continue Reading

Citations

Jun 17, 2010·Surgical Endoscopy·W J A BrokelmanM M P J Reijnen
Nov 26, 1999·Kidney International·P W PeakeJ A Charlesworth
Jun 29, 2010·Journal of Laparoendoscopic & Advanced Surgical Techniques. Part a·Mare M A LensveltMichel M P J Reijnen
Jun 23, 2015·Journal of Functional Biomaterials·Desiree ' LyonDimitrios Karamichos
Sep 29, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Shomron Ben-Horin, Ilan Bank
Jul 19, 2005·Cytokine & Growth Factor Reviews·Weiliang XiaC Yan Cheng
May 28, 2003·Biochemical and Biophysical Research Communications·Naoki MatsumotoYoshiki Nishizawa
Nov 21, 2015·BioMed Research International·Sharan Malagobadan, Noor Hasima Nagoor
Aug 24, 1999·Journal of Cellular Physiology·S L BellisE A Friedman
Mar 16, 2017·Journal of Cellular Physiology·Yoshiaki YamanakaPeter C Amadio
Sep 10, 2010·American Journal of Physiology. Renal Physiology·Andrea HartnerKarl F Hilgers
Nov 14, 2001·Current Opinion in Nephrology and Hypertension·O V SakharovaB M Brenner
Nov 10, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·K J KelynackG J Becker
Sep 9, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·E AlexopoulosM Papadimitriou
Mar 3, 2007·Surgical Endoscopy·Walter J A BrokelmanMichael M P J Reijnen
Mar 6, 2004·The Journal of Medical Investigation : JMI·Shoji Kagami, Shuji Kondo
Jun 17, 2020·Archives of Physiology and Biochemistry·Gordon A FernsMohammad-Hassan Arjmand
Aug 5, 2000·American Journal of Physiology. Renal Physiology·J A Kreidberg, J M Symons
Oct 28, 2017·Scientific Reports·Lei QiangYu-Ying He
Feb 22, 2003·Journal of the American Society of Nephrology : JASN·Benjamin S WestonRoger M Mason
Oct 20, 1999·The American Journal of Medicine·S Adler, H R Brady
Dec 28, 1999·Microbes and Infection·M H Branton, J B Kopp
Jul 20, 2001·Journal of the American Society of Nephrology : JASN·Shuji KondoYasuhiro Kuroda
Dec 9, 1997·The Journal of Laboratory and Clinical Medicine·M A Haralson
Jun 29, 2021·Biophysics Reviews·Pearlin Hameed, Geetha Manivasagam

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.