Transforming growth factor beta (TGFB) signaling is activated during porcine implantation: proposed role for latency-associated peptide interactions with integrins at the conceptus-maternal interface

Reproduction : the Official Journal of the Society for the Study of Fertility
Dana A MassutoLaurie A Jaeger

Abstract

The process of implantation is mediated by a complex network of signaling and adhesive factors. In the pig, latent and active transforming growth factor beta (TGFB), TGFB receptors (TGFBR), and integrins (ITGs) are present during the peri-implantation period. TGFB signals via TGFBR and activates downstream effector SMAD proteins 2 and 3 (p-SMAD2/3). Latency-associated peptide (LAP), part of the latent TGFB complex, is known to bind to ITG heterodimers and activate TGFB. We hypothesize that active TGFBs and TGFBRs along with LAP and ITGs functionally interact at the conceptus-maternal interface to mediate events essential for conceptus development and attachment in pigs. Uteri and conceptuses from days 10, 12, 16, 20, and 24 pregnant gilts were immunostained for TGFB, LAP, and ITG subunits (ITGAV, ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8). Activation of TGFBRs was evaluated by the presence of phosphorylated downstream effector SMAD2/3. Binding of LAP to ITGs was also evaluated using porcine trophectoderm cells. Abundant active TGFB was detected at the apical surfaces of epithelia at the conceptus-maternal interface, and p-SMAD2/3 was detected at both conceptus attachment and nonattachment sites during implantation. Separate aggrega...Continue Reading

References

Aug 1, 1991·Journal of Cellular Physiology·C H Graham, P K Lala
Apr 1, 1990·The Journal of Cell Biology·R M LyonsH L Moses
Oct 1, 1984·Journal of Cellular Physiology·D A LawrenceP Jullien
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·F Chen, R A Weinberg
Aug 4, 1994·Nature·J L WranaJ Massagué
Feb 1, 1994·The Journal of Clinical Investigation·M H Barcellos-HoffJ A Weatherbee
Jan 1, 1996·Annual Review of Cell and Developmental Biology·E Ruoslahti
Apr 1, 1997·Pediatrics in Review·I G De Plaen
Oct 6, 1997·The EMBO Journal·A NakaoP ten Dijke
Oct 23, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E J EhrhartM H Barcellos-Hoff
Mar 26, 1999·Molecular Reproduction and Development·S KallapurT Doetschman
Mar 10, 2000·Cytokine & Growth Factor Reviews·J E Murphy-Ullrich, M Poczatek
Feb 15, 2001·Microscopy Research and Technique·K KoliJ Keski-Oja
Aug 22, 2001·Biology of Reproduction·G A JohnsonR C Burghardt
Sep 13, 2001·Molecular Biology of the Cell·B HinzC Chaponnier
Feb 1, 2002·FEBS Letters·Justin P AnnesJohn S Munger
Jun 14, 2002·Journal of Reproductive Immunology·Bruce A Lessey
Nov 5, 2002·Journal of Cell Science·Min LuLynn M Schnapp
Dec 12, 2002·Cells, Tissues, Organs·Robert C BurghardtFuller W Bazer
Dec 17, 2002·Journal of Cell Science·Justin P AnnesDaniel B Rifkin
Jan 9, 2003·American Journal of Reproductive Immunology : AJRI·Subhradip Karmakar, Chandana Das
Jun 9, 2004·The Journal of Cell Biology·Justin P AnnesDaniel B Rifkin
Jul 13, 2004·Biochimica Et Biophysica Acta·Michele A WozniakPatricia J Keely
Nov 27, 2004·Trends in Cell Biology·Jorma Keski-OjaHarald von Melchner

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Citations

Oct 5, 2010·Human Reproduction·G GiritharanP F Rinaudo
Dec 2, 2014·Journal of Animal Science and Biotechnology·Rodney D GeisertDaniel J Mathew
Sep 1, 2011·Reproduction in Domestic Animals = Zuchthygiene·A J ZiecikA Andronowska
Nov 26, 2010·Molecular Reproduction and Development·Le Ann BlombergRobert W Li
Aug 1, 2012·Asian-Australasian Journal of Animal Sciences·Mingoo KimHakhyun Ka
Dec 13, 2016·International Journal of Molecular Sciences·Liu TengMei Yu
Feb 22, 2013·Biology of Reproduction·Anastazia SamborskiStefan Bauersachs
Apr 11, 2017·Molecular Reproduction and Development·Rodney D GeisertThomas E Spencer
Aug 21, 2012·Reproductive Sciences·Signe AltmäeAndres Salumets
Feb 12, 2015·Journal of Animal Science and Biotechnology·Greg A JohnsonFuller W Bazer
Jun 20, 2017·Molecular Reproduction and Development·Agnieszka WaclawikAdam J Ziecik
Jul 26, 2011·International Journal of Immunopathology and Pharmacology·M MartinelliL Scapoli
Jun 19, 2019·World Journal of Gastroenterology : WJG·Sheng WangSi-Yu Sun

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